Difference between revisions of "Part:BBa K1051200"
 
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<br />
 
<br />
 
<h3>Principle</h3>
 
<h3>Principle</h3>
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'''Ubiquitin''',a small regulatory protein that has been found in almost all tissues of eukaryotic organisms,Ubiquitination is a post translational modification where ubuiquitin is attached to a substrate protein.It is carried out in three main steps; activation, conjugation and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).<br />
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We want to install ubiquitin to fluorescent proteins at N-terminal.<br />
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To test the function of ubiquitin fusion protein, we construct measurement pathway K1051288, which contains J63006, K1051200,K1051003 and K1051006.
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http://upload.wikimedia.org/wikipedia/commons/thumb/5/5b/Ubiquitylation.svg/800px-Ubiquitylation.svg.png
 
http://upload.wikimedia.org/wikipedia/commons/thumb/5/5b/Ubiquitylation.svg/800px-Ubiquitylation.svg.png
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<br />
 
The ubiquitination system.
 
The ubiquitination system.
 +
<br />
 +
'''Ubiquitin''',a small regulatory protein that has been found in almost all tissues of eukaryotic organisms,Ubiquitination is a post translational modification where ubuiquitin is attached to a substrate protein.It is carried out in three main steps; activation, conjugation and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).<br />
 +
We want to install ubiquitin to fluorescent proteins at N-terminal.<br />
 +
To test the function of ubiquitin fusion protein, we construct measurement pathway K1051288, which contains J63006, K1051200,K1051003 and K1051006.
 
https://static.igem.org/mediawiki/2013/6/68/Growth-Curve.jpg
 
https://static.igem.org/mediawiki/2013/6/68/Growth-Curve.jpg
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<br />
 
Yeast growth curve.
 
Yeast growth curve.
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https://static.igem.org/mediawiki/2013/3/3f/Sc_bright_20.jpg
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<br />
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We made the chip as a platform for watching and synchronize the cells. First step is capture the cells by the chip. As showed in the figure, budding yeast can be captured by the chip successfully.
 
<!-- Add more about the biology of this part her
 
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===Usage and Biology===
 
===Usage and Biology===
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<partinfo>BBa_K1051200 parameters</partinfo>
 
<partinfo>BBa_K1051200 parameters</partinfo>
 
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<h3>References</h3>
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[1] Wilkinson KD (October 2005). "The discovery of ubiquitin-dependent proteolysis". Proc. Natl. Acad. Sci. U.S.A. 102 (43): 15280–2. doi:10.1073/pnas.0504842102. PMC 1266097. PMID 16230621.
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<br />
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[2] Kimura Y, Tanaka K (2010). "Regulatory mechanisms involved in the control of ubiquitin homeostasis". J Biochem 147 (6): 793–8. doi:10.1093/jb/mvq044. PMID 20418328.

Latest revision as of 18:32, 27 September 2013

Ubiquitin

Purpose

Works for the degradation of fluorescent proteins we used.

Principle


http://upload.wikimedia.org/wikipedia/commons/thumb/5/5b/Ubiquitylation.svg/800px-Ubiquitylation.svg.png
The ubiquitination system.
Ubiquitin,a small regulatory protein that has been found in almost all tissues of eukaryotic organisms,Ubiquitination is a post translational modification where ubuiquitin is attached to a substrate protein.It is carried out in three main steps; activation, conjugation and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).
We want to install ubiquitin to fluorescent proteins at N-terminal.
To test the function of ubiquitin fusion protein, we construct measurement pathway K1051288, which contains J63006, K1051200,K1051003 and K1051006. Growth-Curve.jpg
Yeast growth curve. Sc_bright_20.jpg
We made the chip as a platform for watching and synchronize the cells. First step is capture the cells by the chip. As showed in the figure, budding yeast can be captured by the chip successfully.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 25
    Illegal AgeI site found at 253
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Wilkinson KD (October 2005). "The discovery of ubiquitin-dependent proteolysis". Proc. Natl. Acad. Sci. U.S.A. 102 (43): 15280–2. doi:10.1073/pnas.0504842102. PMC 1266097. PMID 16230621.
[2] Kimura Y, Tanaka K (2010). "Regulatory mechanisms involved in the control of ubiquitin homeostasis". J Biochem 147 (6): 793–8. doi:10.1093/jb/mvq044. PMID 20418328.