Difference between revisions of "Part:BBa K1051800"

Latest revision as of 19:01, 27 September 2013

protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.

Protein dCas9 is the key of the technology CRISPRi,and this part is the gene of the dcas9. Deleting its ending codon is to provide a convenient way to add other protein domain to improve the function of dCas9.

This part is used with sgRNA(k1051801,k1051802). Principle of CRISPRi is below

By the way, wo should use inducible promoter is regulate it.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2740
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2886
Illegal BsaI site found at 3740
Illegal BsaI.rc site found at 1411

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1051800 short</partinfo>
+      <p>Protein dCas9 is the key of the technology CRISPRi,and this part is the gene of the dcas9. Deleting its ending codon is to provide a convenient way to add other protein domain to improve the function of dCas9.</p>
-Protein dCas9 is the key of the technology CRISPRi,and this part is the gene of the dcas9.Delete its ending codon is to provide an convenient way to add other protein domain to improve the function of dCas9.
+      <p>This part is used with sgRNA(k1051801,k1051802). Principle of CRISPRi is below</p>
+<!-- Add more about the biology of this part here -->
+       https://static.igem.org/mediawiki/parts/1/13/CRISPRi_principle.png
+<br/>By the way, wo should use inducible promoter is regulate it.
-<!-- Add more about the biology of this part here
-      <p>Technology CRISPRi is a very single method to regulate a target gene.</p>
-      <img src="https://static.igem.org/mediawiki/parts/thumb/1/13/CRISPRi_principle.png/120px-CRISPRi_principle.png" >/
 ===Usage and Biology===