Difference between revisions of "Part:BBa K1051115"
 
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<partinfo>BBa_K1051115 parameters</partinfo>
 
<partinfo>BBa_K1051115 parameters</partinfo>
 
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===principle===
 
===principle===
 
<p>The ZRC1 gene encodes a multicopy suppressor of zinc toxicity in Saccharomyces cerevisiae; however, previously it was reported that the expression of ZRC1 was induced when the intracellular zinc level was decreased. The COT1 and ZRC1 genes of Saccharomyces cerevisiae are structurally related dosage-dependent suppressors of metal toxicity. COT1 confers increased tolerance to high levels of cobalt; ZRC1 confers increased tolerance(Conklin, Culbertson, & Kung, 1994).Zrc1 has six putative trans-membrane domains, and Zrc1-GFP fusion protein was localized to the vacuolar membrane. Zrc1 function as a mechanism to maintain the zinc homeostasis in yeast(Miyabe, Izawa, & Inoue, 2001).</p>
 
<p>The ZRC1 gene encodes a multicopy suppressor of zinc toxicity in Saccharomyces cerevisiae; however, previously it was reported that the expression of ZRC1 was induced when the intracellular zinc level was decreased. The COT1 and ZRC1 genes of Saccharomyces cerevisiae are structurally related dosage-dependent suppressors of metal toxicity. COT1 confers increased tolerance to high levels of cobalt; ZRC1 confers increased tolerance(Conklin, Culbertson, & Kung, 1994).Zrc1 has six putative trans-membrane domains, and Zrc1-GFP fusion protein was localized to the vacuolar membrane. Zrc1 function as a mechanism to maintain the zinc homeostasis in yeast(Miyabe, Izawa, & Inoue, 2001).</p>
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===Results===
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https://static.igem.org/mediawiki/2013/f/f1/Vm.jpg
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===Reference===
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Conklin, D. S., Culbertson, M. R., & Kung, C. (1994). Interactions between gene products involved in divalent cation transport in Saccharomyces cerevisiae. Molecular and General Genetics MGG, 244(3), 303-31
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Miyabe, S., Izawa, S., & Inoue, Y. (2001). The Zrc1 Is Involved in Zinc Transport System between Vacuole and Cytosol in< i> Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications, 282(1), 79-83

Latest revision as of 04:06, 3 October 2013

ZRC1;Vacuolar Membrane Targeting Protein

After added with 23 prefix and suffix, it can be used as a transporter to the vacuolar membrane of baking yeast. Also, when joining with different flourescent proteins, they can light the inner membrane using such proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 52
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 55
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 52
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 52
  • 1000
    COMPATIBLE WITH RFC[1000]


principle

The ZRC1 gene encodes a multicopy suppressor of zinc toxicity in Saccharomyces cerevisiae; however, previously it was reported that the expression of ZRC1 was induced when the intracellular zinc level was decreased. The COT1 and ZRC1 genes of Saccharomyces cerevisiae are structurally related dosage-dependent suppressors of metal toxicity. COT1 confers increased tolerance to high levels of cobalt; ZRC1 confers increased tolerance(Conklin, Culbertson, & Kung, 1994).Zrc1 has six putative trans-membrane domains, and Zrc1-GFP fusion protein was localized to the vacuolar membrane. Zrc1 function as a mechanism to maintain the zinc homeostasis in yeast(Miyabe, Izawa, & Inoue, 2001).

Results

Vm.jpg

Reference

Conklin, D. S., Culbertson, M. R., & Kung, C. (1994). Interactions between gene products involved in divalent cation transport in Saccharomyces cerevisiae. Molecular and General Genetics MGG, 244(3), 303-31 Miyabe, S., Izawa, S., & Inoue, Y. (2001). The Zrc1 Is Involved in Zinc Transport System between Vacuole and Cytosol in< i> Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications, 282(1), 79-83