Difference between revisions of "Part:BBa K1024000"
 
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<b>Design:</b> We constructed and improved standard BioBrick Parts about quorum sensing systems and modified the systems in prokaryotic microorganisms for usage in S. cerevisiae (Yeast). We modified the transcription activator, LuxR (BBa_C0062), by adding nuclear localization signal (NLS) sequence and Herpes simplex virus VP16 activation domain in N-terminus of LuxR, and ligate the sequence of this modified LuxR downstream TEF promoter, which is the constitutive promoter in yeast (BBa_K1024000).</P>
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<b>Design:</b> We constructed and improved standard BioBrick Parts about quorum sensing systems and modified the systems in prokaryotic microorganisms for usage in S. cerevisiae (Yeast). We modified the transcription activator, LuxR (BBa_C0062), by adding nuclear localization sequence (NLS) and Herpes simplex virus VP16 activation domain in N-terminus of LuxR, and ligate the sequence of this modified LuxR downstream TEF promoter, which is the constitutive promoter in yeast (BBa_K1024000).</P>
  
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https://static.igem.org/mediawiki/igem.org/6/6a/Tsinghua-BB-000.png
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<b>Figure 1. The principle of quorum sensing</b><br>
 
===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 20:29, 27 September 2013

BBa_K1024000


LuxR in Yeast (pTEF+VP16+NLS+LuxR)

Part Name: BBa_K1024000

Short Description: LuxR in Yeast (pTEF+VP16+NLS+LuxR)

Part Type: Regulatory

Design: We constructed and improved standard BioBrick Parts about quorum sensing systems and modified the systems in prokaryotic microorganisms for usage in S. cerevisiae (Yeast). We modified the transcription activator, LuxR (BBa_C0062), by adding nuclear localization sequence (NLS) and Herpes simplex virus VP16 activation domain in N-terminus of LuxR, and ligate the sequence of this modified LuxR downstream TEF promoter, which is the constitutive promoter in yeast (BBa_K1024000).

Tsinghua-BB-0000.png

Figure 1. The principle of quorum sensing

Usage and Biology

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1580
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 506
    Illegal BsaI site found at 893
    Illegal BsaI site found at 1280
    Illegal BsaI.rc site found at 547
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 1321