Difference between revisions of "Part:BBa K1024005"
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<b>Design:</b> The part was the reporter of the pathogen detector. Tet operator and CYC1 TATA region was followed by ADE2 gene. In the yeast S. cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Mated with sensor yeast (BBa_K1024004) which is induced by AHL, the ADE2 gene will be activated and rescuing the ADE2 knockout yeast by making it white. | <b>Design:</b> The part was the reporter of the pathogen detector. Tet operator and CYC1 TATA region was followed by ADE2 gene. In the yeast S. cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Mated with sensor yeast (BBa_K1024004) which is induced by AHL, the ADE2 gene will be activated and rescuing the ADE2 knockout yeast by making it white. | ||
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| + | https://static.igem.org/mediawiki/igem.org/a/a5/Tsinghua-BB-005.png | ||
| + | <b>Figure 1. The model of reporter yeast </b><br> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 20:31, 27 September 2013
Report yeast (inducible ADE2)
Part Name: BBa_K1024005
Short Description: Report yeast (inducible ADE2)
Part Type: Signaling
Design: The part was the reporter of the pathogen detector. Tet operator and CYC1 TATA region was followed by ADE2 gene. In the yeast S. cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Mated with sensor yeast (BBa_K1024004) which is induced by AHL, the ADE2 gene will be activated and rescuing the ADE2 knockout yeast by making it white.
Figure 1. The model of reporter yeast
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1053
Illegal BamHI site found at 456 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]