Difference between revisions of "Part:BBa K1033222:Design"
 
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===Design Notes===
 
===Design Notes===
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This part is designed with the same sticky ends as you would get by cutting it with EcoRI-HF and Spel.
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This part was based on a consensus sequence found in ''Lactococcus lactis''. It was synthesized by using primers containing a overhang with the standard prefix and suffix.
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===Source===
 
===Source===

Latest revision as of 17:25, 4 October 2013

Promoter CP29


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was based on a consensus sequence found in Lactococcus lactis. It was synthesized by using primers containing a overhang with the standard prefix and suffix.

Source

This part is a synthetic promoter based on a promoter library from the article "The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters." by Peter Ruhdal Jensen and Karin Hammer.

References

P. Jensen, K Hammer Appl: The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters Environ Microbiol. 64.1 (1998) 82–87