Difference between revisions of "Part:BBa K1124008:Design"
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===Design Notes===
 
===Design Notes===
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This part was generated by site-directed mutagenesis on micC-sRNA scaffold([[BBa_K1124005]]), in which target-binding sequence was added to the scaffold.
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This part was generated by site-directed mutagenesis on MicC-sRNA scaffold([[BBa_K1124005]]), in which the target-binding sequence was added to the scaffold.
−
Target binding sequence was designed complementary to the hycA gene of ''E. coli'' K-12 strain.
+
The target binding sequence was designed complementary to the hycA gene of ''E. coli'' K-12 strain.
  
  
 
===Source===
 
===Source===
−
 
+
We designed oligo DNA complementary to the first 24nt of hycA CDS as the target binding sequence. Then, we integrated it with MicC sRNA scaffold ([[BBa_K1124005]]) using PCR technique.
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PCR
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===References===
 
===References===
 +
Viviana Sanchez-Torres,1 Toshinari Maeda,and Thomas K.(2009). Wood -Protein Engineering of the Transcriptional Activator FhlA To Enhance Hydrogen Production in Escherichia coli- APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept.  5639–5646
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Yoo, S. M., Na, D., & Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocols, 8(9), 1694-1707.

Latest revision as of 14:10, 27 September 2013

anti-hycA sRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was generated by site-directed mutagenesis on MicC-sRNA scaffold(BBa_K1124005), in which the target-binding sequence was added to the scaffold. The target binding sequence was designed complementary to the hycA gene of E. coli K-12 strain.


Source

We designed oligo DNA complementary to the first 24nt of hycA CDS as the target binding sequence. Then, we integrated it with MicC sRNA scaffold (BBa_K1124005) using PCR technique.

References

Viviana Sanchez-Torres,1 Toshinari Maeda,and Thomas K.(2009). Wood -Protein Engineering of the Transcriptional Activator FhlA To Enhance Hydrogen Production in Escherichia coli- APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept. 5639–5646

Yoo, S. M., Na, D., & Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocols, 8(9), 1694-1707.