Difference between revisions of "Part:BBa K1111001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | |||
| − | |||
| + | The Promoter was isolated directly from the Genimic DNA by PCR. In order to have the entire sequence, we took the sequence 500bp upstream until the start codon. | ||
===Source=== | ===Source=== | ||
| − | + | Escherichia Coli, K-12, Substrain MG1655 | |
===References=== | ===References=== | ||
| − | Journal of Bacteriology | + | Journal of Bacteriology <BR> |
| − | Response of hya Expression to External pH in Escherichia coli | + | Response of hya Expression to External pH in Escherichia coli <BR> |
| − | Paul W. King and Alan E. Przybyla | + | Paul W. King and Alan E. Przybyla <BR> |
| − | J.Bacteriol. 1990 | + | J.Bacteriol. 1990 <BR> |
| + | |||
| − | Journal of Bacteriology | + | Journal of Bacteriology <BR> |
| − | Mutational analysis and charecterization of the Escherichia coli hya operin, which encodes [NiFe] hydrogenase1 | + | Mutational analysis and charecterization of the Escherichia coli hya operin, which encodes [NiFe] hydrogenase1 <BR> |
| − | N K Menon, J Robbins, J C Wendt, K T Shanmuagam and A E Przybyla | + | N K Menon, J Robbins, J C Wendt, K T Shanmuagam and A E Przybyla <BR> J. Bacteriol 1991 |
Latest revision as of 12:49, 27 September 2013
Hya promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 184
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 184
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 184
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 184
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 184
Illegal AgeI site found at 26 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Promoter was isolated directly from the Genimic DNA by PCR. In order to have the entire sequence, we took the sequence 500bp upstream until the start codon.
Source
Escherichia Coli, K-12, Substrain MG1655
References
Journal of Bacteriology
Response of hya Expression to External pH in Escherichia coli
Paul W. King and Alan E. Przybyla
J.Bacteriol. 1990
Journal of Bacteriology
Mutational analysis and charecterization of the Escherichia coli hya operin, which encodes [NiFe] hydrogenase1
N K Menon, J Robbins, J C Wendt, K T Shanmuagam and A E Przybyla
J. Bacteriol 1991