Difference between revisions of "Part:BBa K1228006"

(→‎Reference)
(→‎Experiment and Results)
 
(27 intermediate revisions by 3 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_K1228006 short</partinfo>
 
<partinfo>BBa_K1228006 short</partinfo>
  
−
Lf (loctoferrin) is located in many key body fluids , e.g., milk, tears, saliva and other secretions, and in white blood cells ,it has become clear that it is an important part of mammalian defence mechanisms, with a wide range of protective functions.25 amino acid residues is from Lf but has a strong bactericidal, bacteriostatic ability, and the antimicrobial activity than natural LF.We add T1 terminator and Constitutive promoter veg on the 25aa to make it can  work regularly.
+
This generator contains BBa_K541503(https://parts.igem.org/Part:BBa_K541503) and BBa_K1228005(https://parts.igem.org/Part:BBa_K1228005). The generator can secrete 25 aminoacid peptide that is a part of Lactoferrin in ''B.subtilis''. It has a broad anti-bacterial range and strong anti-bacterial activity.
  
  
Line 17: Line 17:
 
<partinfo>BBa_K1228006 parameters</partinfo>
 
<partinfo>BBa_K1228006 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
== Experiment and Results ==
 +
<p>1. Inoculate recombinant strains into 20ml LB.Cultivate at 37°C overnight.</p>
 +
<p>2. Add 200ul indicator inoculum (''Micrococcus Lysodeikticus'' or ''Escherichia Coli'') untill heated LB solid medium (100ml) cool to about 50°C.</p>
 +
<p>3. Use puncher make several hole on the LB plate.Add 80ul recombinant strains inoculum into the hole.Cultivate at 37°C overnight.</p>
 +
4. Observe whether LB plate will appear inhibition zone.
 +
 +
 +
<html>
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 +
<style type="text/css">
 +
.zoomPan{width:800px;position:relative; }
 +
.sh{zoom:1;background:url(https://static.igem.org/mediawiki/2013/8/80/Old_wall.jpg);filter:progid:DXImageTransform.Microsoft.dropShadow(color='#54000000', OffX=2,OffY=2);-webkit-box-shadow:4px 4px 4px #666;-moz-box-shadow:4px 4px 4px #666;}
 +
#zoom{position:absolute;width:254px;height:254px;border:3px solid #fff;left:-9999px;top:0;overflow:hidden;background:#fff;}
 +
#zoom img{position:relative;}
 +
</style>
 +
</head>
 +
 +
<script type="text/javascript">
 +
//<![CDATA[
 +
function zoomBox() {this.index.apply(this, arguments)}
 +
zoomBox.prototype = {
 +
    index: function(win,zoom) {
 +
        var win=document.getElementById(win);
 +
        var box=document.getElementById(zoom);
 +
        var img=box.getElementsByTagName('IMG')[0];
 +
        var zoom=img.width/win.getElementsByTagName('IMG')[0].width;
 +
        var z=Math.round(box.offsetWidth/2);
 +
        win.onmousemove=function (e){
 +
            e = e || window.event;
 +
            var x=e.clientX,y=e.clientY, ori=win.getBoundingClientRect();
 +
            if (x>ori.right+20||y>ori.bottom+20||x<ori.left-20||y<ori.top-20)box.style.display='none';
 +
            x-=ori.left;
 +
            y-=ori.top;
 +
            box.style.left=x-z+'px';
 +
            box.style.top=y-z+'px';
 +
            img.style.left=-x*zoom+z+'px';
 +
            img.style.top=-y*zoom+z+'px';
 +
        }
 +
        win.onmouseover=function (){box.style.display=''}
 +
    }
 +
};
 +
window.onload=function (){
 +
    x=new zoomBox('zoomPan','zoom')
 +
}
 +
//]]>
 +
</script>
 +
 +
 +
 +
<div id="paragraphs">
 +
        <div class="zoomPan" id="zoomPan">
 +
        <img src="https://static.igem.org/mediawiki/2013/6/64/ZswM21S.jpg" width="800px;">
 +
        <div id="zoom" class="sh"><img src="https://static.igem.org/mediawiki/2013/1/16/ZswM21b.jpg" width="1500px;"></div>
 +
        </div>
 +
    </div>
 +
</html>
 +
 +
https://static.igem.org/mediawiki/2013/4/46/ZswM21_M22s.jpg
  
 
== Reference ==
 
== Reference ==
−
BellamyW,TakaseM,Yamauchi K,et al. Identification of the bactericidal domainof lactoferrin[J]. Biochimicaet BiophysicaActa,1992,1121( 1/2):130-136.
+
<p>1.BellamyW,TakaseM,Yamauchi K,et al. Identification of the bactericidal domainof lactoferrin[J]. BiochimicaetBiophysicaActa,1992,1121( 1/2):130-136.</p>
−
EliassenLT,Berge G,SveinbjornssonB,et al. Evidenceforadirect antitumormechanismof actionof Bovine lactoferricin[J]． Anticancer Research,2002,22(5):2703－2710.
+
<p>2.EliassenLT,Berge G,SveinbjornssonB,et al. Evidenceforadirect antitumormechanismof actionof Bovinel actoferricin[J].AnticancerResearch,2002,22(5):2703－2710.</p>
−
Wakabayashi H,TakaseM,TomitaM. Lactoferricin derived from milk protein lactoferrin[J]. Current Pharmaceutical Design,2003,9(16):1277－1287.
+
<p>3.Wakabayashi H,TakaseM,TomitaM. Lactoferricin derived from milk protein lactoferrin[J]. Current Pharmaceutical Design,2003,9(16):1277－1287.</p>

Latest revision as of 15:55, 27 September 2013

25aa with T1 terminator and Constitutive promoter veg

This generator contains BBa_K541503(https://parts.igem.org/Part:BBa_K541503) and BBa_K1228005(https://parts.igem.org/Part:BBa_K1228005). The generator can secrete 25 aminoacid peptide that is a part of Lactoferrin in B.subtilis. It has a broad anti-bacterial range and strong anti-bacterial activity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experiment and Results

1. Inoculate recombinant strains into 20ml LB.Cultivate at 37°C overnight.

2. Add 200ul indicator inoculum (Micrococcus Lysodeikticus or Escherichia Coli) untill heated LB solid medium (100ml) cool to about 50°C.

3. Use puncher make several hole on the LB plate.Add 80ul recombinant strains inoculum into the hole.Cultivate at 37°C overnight.

4. Observe whether LB plate will appear inhibition zone.


ZswM21_M22s.jpg

Reference

1.BellamyW,TakaseM,Yamauchi K,et al. Identification of the bactericidal domainof lactoferrin[J]. BiochimicaetBiophysicaActa,1992,1121( 1/2):130-136.

2.EliassenLT,Berge G,SveinbjornssonB,et al. Evidenceforadirect antitumormechanismof actionof Bovinel actoferricin[J].AnticancerResearch,2002,22(5):2703－2710.

3.Wakabayashi H,TakaseM,TomitaM. Lactoferricin derived from milk protein lactoferrin[J]. Current Pharmaceutical Design,2003,9(16):1277－1287.