Difference between revisions of "Part:BBa K1036004"

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This newly found GFP variant Superfolder GFP, which has shown outstanding performance in many ways. It has two advantages in observing on microfluidic:  
 
This newly found GFP variant Superfolder GFP, which has shown outstanding performance in many ways. It has two advantages in observing on microfluidic:  
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1) Fold 3.5 to 4 times faster than regular GFP;
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1)Fold 3.5 to 4 times faster than regular GFP;
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2) Yield up to four times more total Fluorescence than regular GFP.
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2)Yield up to four times more total Fluorescence than regular GFP.
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The greater the fluorescence strength is, the shorter exposure time will be needed, thus can decrease the photobleaching (GFP will be eventually destroyed by the light exposure that tries to stimulate it into fluorescing) that will lead into lapses in data processing.<br/>More detailed characterization was performed by Cambridge iGEM team 2008.<a href="http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP">http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP<a/>
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The greater the fluorescence strength is, the shorter exposure time will be needed, thus can decrease the photobleaching (GFP will be eventually destroyed by the light exposure that tries to stimulate it into fluorescing) that will lead into lapses in data processing.<br/>More detailed characterization was performed by Cambridge iGEM team 2008(<a href="http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP">http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP</a>).
 
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=Experimental Data=
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The Fig.1 and Fig.2 showed the correct result that the LVA-tag was added to the end of <i>sfgfp</i> gene.<br/>
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The Fig.3 showed the difference of GFP and sfGFP.<br/><br/>
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<img src="https://static.igem.org/mediawiki/2013/c/ce/BBa_K1036004-Fig.1.png" width=400px; height=280px;/><br/><br/>
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<img src="https://static.igem.org/mediawiki/2013/9/98/BBa_K1036004-Fig.2.png" width=850px; height=300px;/><br/><br/>
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<img src="https://static.igem.org/mediawiki/2013/0/03/BBa_K1036004-Fig.3.png" width=450px; height=300px;/><br/><br/>
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<!-- Add more about the biology of this part here
 
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Latest revision as of 23:12, 27 September 2013

sfgfp with LVA-tag

Description

This newly found GFP variant Superfolder GFP, which has shown outstanding performance in many ways. It has two advantages in observing on microfluidic: 1)Fold 3.5 to 4 times faster than regular GFP; 2)Yield up to four times more total Fluorescence than regular GFP. The greater the fluorescence strength is, the shorter exposure time will be needed, thus can decrease the photobleaching (GFP will be eventually destroyed by the light exposure that tries to stimulate it into fluorescing) that will lead into lapses in data processing.
More detailed characterization was performed by Cambridge iGEM team 2008(http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP).

Experimental Data

The Fig.1 and Fig.2 showed the correct result that the LVA-tag was added to the end of sfgfp gene.
The Fig.3 showed the difference of GFP and sfGFP.







Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13