Difference between revisions of "Part:BBa K1036005:Design"
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===Design Notes=== | ===Design Notes=== | ||
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− | + | This part contains two subparts: RBS-sfgfp(-LVA)-TT(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036004">BBa_K1036004</a>)and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> (located in 2013-Plate 1-12D). RBS-sfgfp(-LVA)-TT digested by <i>Eco</i>R I & <i>Spe</i>I and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> digested by <i>Eco</i>R I & <i>Xba</i>I were ligated together by T4 ligase. | |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
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+ | 1. <a href="http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP">http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP</a><br/> | ||
+ | 2. <a href="http://www.ncbi.nlm.nih.gov/pubmed/16369541">Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)</a><br/> | ||
+ | 3. <a href="http://www.ncbi.nlm.nih.gov/pubmed?term=A%20sensing%20array%20of%20radically%20coupled%20genetic%20'biopixels'.[all]&cmd=correctspelling">Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)</a> | ||
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Latest revision as of 21:58, 27 September 2013
sfgfp (with LVA-tag) under plux R control
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2737
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 914
Illegal BsaI.rc site found at 2017
Illegal SapI.rc site found at 31
Design Notes
This part contains two subparts: RBS-sfgfp(-LVA)-TT(BBa_K1036004)and BBa_K546000 (located in 2013-Plate 1-12D). RBS-sfgfp(-LVA)-TT digested by EcoR I & SpeI and BBa_K546000 digested by EcoR I & XbaI were ligated together by T4 ligase.
Source
The template of sfgfp gene namely biobrick pSB1C3-RBS-sfgfp-TT was provided by 2013 iGEM team Peking.We add the LVA-tag in the end of sfgfp.
We thanks for Peking's help.
References
1. http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP
2. Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)
3. Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)