Difference between revisions of "Part:BBa K1074000:Experience"
(→User Reviews) |
(→User Reviews) |
||
| (10 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 26: | Line 25: | ||
<I>USTC_China iGEM13</I> | <I>USTC_China iGEM13</I> | ||
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
| − | In our project,we fused TD1 with a varity of antigens(HBsAg(Hepatitis B virus)<partinfo>BBa_K1074002</partinfo>,Ag85b(Mycobacterium tuberculosis)<partinfo>BBa_K1074003</partinfo>,PAD4(Bacillus Anthracis)<partinfo>BBa_K1074004</partinfo> ),adjuvant LTB (<partinfo>BBa_K1074005</partinfo>)and GFP(<partinfo>BBa_E0040</partinfo>), induced expression in BL21 and ''Bacillus Subtilis'' WB800N with IPTG and tested positive by SDS-PAGE and ELISA. Then we validated the efficient transdermal function of TD1 fused antigen HBsAg by ELISA. Students in Wen lab also validated the efficient transdermal function of N-terminal TD1 modified GFP(not BBa_E0040) | + | |
| − | + | In our project,we fused TD1 with a varity of antigens(HBsAg(Hepatitis B virus)<partinfo>BBa_K1074002</partinfo>,Ag85b(Mycobacterium tuberculosis)<partinfo>BBa_K1074003</partinfo>,PAD4(Bacillus Anthracis)<partinfo>BBa_K1074004</partinfo> ),adjuvant LTB (<partinfo>BBa_K1074005</partinfo>)and GFP(<partinfo>BBa_E0040</partinfo>), induced expression in BL21 and ''Bacillus Subtilis'' WB800N with IPTG and tested positive by SDS-PAGE and ELISA. Then we validated the efficient transdermal function of TD1 fused antigen HBsAg by ELISA. Students in Wen lab also validated the efficient transdermal function of N-terminal TD1 modified GFP(not BBa_E0040) | |
| + | [[Image:USTC_China_iGEM13_BBa_K1074000_4.jpg|thumb|left|600px|figure 1 Our Transdermal test device]] | ||
| + | [[Image:USTC_China_iGEM13_BBa_K1074006_5.jpg|thumb|left|600px|figure 2 ELISA test transdermal function of N-terminal TD1 modified antigen HBsAg ]] | ||
| + | |||
| + | |||
|}; | |}; | ||
Latest revision as of 18:01, 27 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1074000
User Reviews
UNIQ3242912053dd1720-partinfo-00000000-QINU UNIQ3242912053dd1720-partinfo-00000001-QINU
|
•••••
USTC_China iGEM13 |
In our project,we fused TD1 with a varity of antigens(HBsAg(Hepatitis B virus)BBa_K1074002,Ag85b(Mycobacterium tuberculosis)BBa_K1074003,PAD4(Bacillus Anthracis)BBa_K1074004 ),adjuvant LTB (BBa_K1074005)and GFP(BBa_E0040), induced expression in BL21 and Bacillus Subtilis WB800N with IPTG and tested positive by SDS-PAGE and ELISA. Then we validated the efficient transdermal function of TD1 fused antigen HBsAg by ELISA. Students in Wen lab also validated the efficient transdermal function of N-terminal TD1 modified GFP(not BBa_E0040)
|
