Difference between revisions of "Part:BBa K1036003:Design"
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| + | We used the biobrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_F2621">BBa_F2621</a> (located in 2012-Plate 2-21F). | ||
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===References=== | ===References=== | ||
Latest revision as of 21:44, 27 September 2013
lux pL controlled luxR with lux pR controlled gfp (LVA-tag)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3909
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
Illegal BsaI.rc site found at 1828
Illegal BsaI.rc site found at 2086
Illegal BsaI.rc site found at 3189
Design Notes
This part contains two subparts: BBa_F2621 (located in 2012-Plate 2-21F) and BBa_K1036002.
BBa_F2621 digested by EcoR & SpeI and BBa_K1036002 digested by EcoR & XbaI were ligated together by T4 ligase.
Source
We used the biobrick BBa_F2621 (located in 2012-Plate 2-21F).
References
Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)