Difference between revisions of "Part:BBa K1036002:Design"

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===Design Notes===
 
===Design Notes===
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This part contains two subparts: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I763020">BBa_I763020</a> (located in 2013-Plate 3-11H) and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> (located in 2013-Plate 1-12D). <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I763020">BBa_I763020</a> digested by <i>Eco</i>R & <i>Spe</i>I and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> digested by <i>Eco</i>R & <i>Xba</i>I were ligated together by T4 ligase.
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===Source===
 
===Source===
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We used the biobrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I763020">BBa_I763020</a> (located in 2013-Plate 3-11H) and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> (located in 2013-Plate 1-12D).
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===References===
 
===References===
 
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<a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=The+identification+of+primary+sites+of+superoxide+and+hydrogen+peroxide+formation+in+the+aerobic+respiratory+chain+and+sulfite+reductase+complex+of+Escherichia+coli.">Messner, K. R. & Imlay, J. A. The identification of primary sites of superoxide and hydrogen peroxide formation in the aerobic respiratory chain and sulfite reductase complex of <i>Escherichia coli</i>. J. Biol. Chem. 274, 10119-10128 (1999)</a>
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<a href="http://www.ncbi.nlm.nih.gov/pubmed?term=A%20sensing%20array%20of%20radically%20coupled%20genetic%20'biopixels'.[all]&cmd=correctspelling">Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)</a>
 
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Latest revision as of 07:02, 27 September 2013


gfp (with LVA-tag) under plux R control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2743
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662
    Illegal BsaI.rc site found at 920
    Illegal BsaI.rc site found at 2023


Design Notes

This part contains two subparts: BBa_I763020 (located in 2013-Plate 3-11H) and BBa_K546000 (located in 2013-Plate 1-12D). BBa_I763020 digested by EcoR & SpeI and BBa_K546000 digested by EcoR & XbaI were ligated together by T4 ligase.

Source

We used the biobrick BBa_I763020 (located in 2013-Plate 3-11H) and BBa_K546000 (located in 2013-Plate 1-12D).

References

Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)