Difference between revisions of "Part:BBa K1073020:Experience"

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===Applications of BBa_K1073020===
 
===Applications of BBa_K1073020===
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When aeBlue is expressed, the colonies on plates as well as the liquid culture become blue. The iGEM Team Braunschweig 2013 used this device to differentiate two different strains from one approach.
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The iGEM Team Braunschweig 2013 used this device as a reporter to distinguish two different strains. <br>
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When aeBlue is expressed, the colonies on plates as well as the liquid culture become blue.
  
 
===User Reviews===
 
===User Reviews===
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<I>iGEM Team Braunschweig 2013</I>
 
<I>iGEM Team Braunschweig 2013</I>
 
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The chromoprotein aeBlue exhibits a distinct blue color when expressed. In order to avoid absorption of the chomoproteins during measurement with the photometer the absorption and emission spectra were measured and an optimal wavelength for spectral optical density measurement was determined according to these spectra.  
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The chromoprotein aeBlue exhibits a distinct blue color when expressed. In order to avoid absorption by the chomoproteins during OD measurement of a bacterial culture, the absorption and emission spectra were measured. An optimal wavelength for spectral optical density measurement was determined to be at 520nm according to these spectra.  
  
 
[[File:AeBlue.jpg |400px]]
 
[[File:AeBlue.jpg |400px]]
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[[File:BS2013Emission aeBlue.jpg |400px]]
 
[[File:BS2013Emission aeBlue.jpg |400px]]
  
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'''iGEM Team Braunschweig 2013:'''Emission spectrum of chromoprotein aeBlue. This chromoprotein does not show any significant emission in a wavelength range between 500-800 nm and therefore cannot be detected by its fluorescence.
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'''iGEM Team Braunschweig 2013:'''Emission spectrum of the chromoprotein aeBlue. This chromoprotein does not show any significant emission in a wavelength range between 500-800 nm and therefore cannot be detected by its fluorescence.
  
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'''Emission maximum of aeBlue:''' none (dark quencher)
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'''Emission maximum of aeBlue:''' none  
  
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AeBlue is a so called dark quencher protein. When excited this kind of chromophores relax back to the ground state without emitting energy through radiation and remain dark [Le Reste et al., 2012].  
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Since the iGEM Team Braunschweig could not detect any emission when prufified aeBlue was excited by UV-light it is possible that this chromoprotein is a so called dark quencher protein. When excited, this kind of chromophores relax back to the ground state without emitting energy through radiation and remain dark [Le Reste et al., 2012].  
  
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According to these results the optical density for growth curves of cells expressing this protein was measured at 520 nm with respect to the chromoproteins amilGFP (<partinfo>BBa_K1073024</partinfo>) and eforRed (<partinfo>BBa_K1073022</partinfo>) to avoid absorption by the proteins. Comparing all absorption spectra they all show an absorption minimum at 520 nm.
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The optical density of cultures with cells expressing aeBlue was measured at a wavelength of 520 nm to avoid errors in measurement through absorption by the protein.
 
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Latest revision as of 14:21, 30 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1073020

The iGEM Team Braunschweig 2013 used this device as a reporter to distinguish two different strains.
When aeBlue is expressed, the colonies on plates as well as the liquid culture become blue.

User Reviews

UNIQ705c2b31e70a0920-partinfo-00000000-QINU

•••••

iGEM Team Braunschweig 2013

The chromoprotein aeBlue exhibits a distinct blue color when expressed. In order to avoid absorption by the chomoproteins during OD measurement of a bacterial culture, the absorption and emission spectra were measured. An optimal wavelength for spectral optical density measurement was determined to be at 520nm according to these spectra.

AeBlue.jpg

iGEM Team Braunschweig 2013: Absorption spectrum of chromoprotein aeBlue in a wavelength range of 400-800 nm.

Absorption maximum of aeBlue: 595 nm

BS2013Emission aeBlue.jpg

iGEM Team Braunschweig 2013:Emission spectrum of the chromoprotein aeBlue. This chromoprotein does not show any significant emission in a wavelength range between 500-800 nm and therefore cannot be detected by its fluorescence.

Emission maximum of aeBlue: none

Since the iGEM Team Braunschweig could not detect any emission when prufified aeBlue was excited by UV-light it is possible that this chromoprotein is a so called dark quencher protein. When excited, this kind of chromophores relax back to the ground state without emitting energy through radiation and remain dark [Le Reste et al., 2012].

The optical density of cultures with cells expressing aeBlue was measured at a wavelength of 520 nm to avoid errors in measurement through absorption by the protein.

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UNIQ705c2b31e70a0920-partinfo-00000002-QINU