Difference between revisions of "Part:BBa K1073013:Experience"

(→‎Applications of BBa_K1073013)
(→‎User Reviews)
 
(One intermediate revision by one other user not shown)
Line 13: Line 13:
 
|-
 
|-
 
|width='10%'|
 
|width='10%'|
−
<partinfo>BBa_K1073013</partinfo>
+
<partinfo>BBa_K1073013 AddReview 4</partinfo>
 
<I>iGEM Team Braunschweig 2013</I>
 
<I>iGEM Team Braunschweig 2013</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
−
This device was successfully employed in BBa_K1073035 to mediate ampicillin resistance in the presence of LasR and N-3-oxododecanoyl-HSL. Due to the leakiness of the promoter cells also grow when N-3-oxododecanoyl-HSL is not present. Uninduced activity of the promoter differs depending on strain and media used.
+
This device was successfully employed in <partinfo>BBa_K1073034</partinfo> to mediate ampicillin resistance in the presence of LasR and N-3-oxododecanoyl-HSL. Due to a low level leakiness of the promoter and the bacteriostatic properties of ampicillin, the cells will start to grow in batch cultivations when a low level of ampicillin is reached even whithoug N-buturyl-HSL. Uninduced activity of the promoter differs depending on strain and media used.
 
|};
 
|};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K1073013 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K1073013 EndReviews</partinfo>

Latest revision as of 11:03, 28 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1073013

iGEM Team Braunschweig 2013 used this device for the construction of BBa_K1073034. It was used in order to induce growth in the presence of LasR and N-3-oxododecanoyl-HSL in ampicillin containing media.

User Reviews

UNIQ38bf86ec94c39ad1-partinfo-00000001-QINU

••••

iGEM Team Braunschweig 2013

This device was successfully employed in BBa_K1073034 to mediate ampicillin resistance in the presence of LasR and N-3-oxododecanoyl-HSL. Due to a low level leakiness of the promoter and the bacteriostatic properties of ampicillin, the cells will start to grow in batch cultivations when a low level of ampicillin is reached even whithoug N-buturyl-HSL. Uninduced activity of the promoter differs depending on strain and media used.

;

UNIQ38bf86ec94c39ad1-partinfo-00000004-QINU