Difference between revisions of "Part:BBa K1139018:Design"

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===Design Notes===
 
===Design Notes===
−
This biobrick is derived from M13mp18 phage vector, amplified by using PCR.
+
This biobrick is derived from M13mp18 phage vector (NEB), amplified by using PCR.
  
 
===Source===
 
===Source===
 
Fwd primer:5'-ACGTGAATTCGCGGCCGCTTCTAGAGAACCGGGGTACATATGATTGAC-3'<br>
 
Fwd primer:5'-ACGTGAATTCGCGGCCGCTTCTAGAGAACCGGGGTACATATGATTGAC-3'<br>
 
Rev primer:5'-CTGCAGCGGCCGCTACTAGTACCTGTGTGAAATTGTTATCCGC-3'<br>
 
Rev primer:5'-CTGCAGCGGCCGCTACTAGTACCTGTGTGAAATTGTTATCCGC-3'<br>
 +
 +
Sequence information is from:
 +
http://www.ncbi.nlm.nih.gov/nuccore/M77815.1
 +
<br>We confirmed that the sequence of the submitted part was a little different from described.
  
 
===References===
 
===References===

Latest revision as of 03:35, 28 September 2013

Promoterless-M13 + Plac on pSB3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 100
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6026
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick is derived from M13mp18 phage vector (NEB), amplified by using PCR.

Source

Fwd primer:5'-ACGTGAATTCGCGGCCGCTTCTAGAGAACCGGGGTACATATGATTGAC-3'
Rev primer:5'-CTGCAGCGGCCGCTACTAGTACCTGTGTGAAATTGTTATCCGC-3'

Sequence information is from: http://www.ncbi.nlm.nih.gov/nuccore/M77815.1
We confirmed that the sequence of the submitted part was a little different from described.

References

1. Atsushi Higashitani. Nahoko Higashitani. Kensuke Horiuchi, DNA Replication in Filamentous Bacteriophage, Protein, Nucleic Acid and Enzyme(1994), vol39:2189-2197.
2. Kensuke Horiuchi, Origin of DNA replication of filamentous coliphages, Jpn. J. Genet(1990), vol65:225-241.
3. ME Ortiz. D Endy., Engineered cell-cell communication via DNA messaging, J. Biol. Engineering(2012), 6-16.
4. A Goñi-Moreno, M Amos, F de la Cruz, Multicellular Computing Using Conjugation for Wiring, PLOS ONE(2013), Vol8.:Issue6:e65986.
5. M Shintani. N Fukushima. M Tezuka. H Yamane. H Nojiri, Conjugative transfer of the IncP-7 carbazole degradative plasmid, pCAR1, in river water samples, Biotechnol Lett (2008). vol30:117-122
6.H Nojiri Structural and Molecular Genetic Analyses of the Bacterial Carbazole Degradation System Biosci, Biotechnol, Biochem, 76(1), 1-18, 2012