Difference between revisions of "Part:BBa K1084124:Design"
 
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===Design Notes===
 
===Design Notes===
−
This RBS has an sequence called an "enhancer". The enhancer is located upstream of the SD seqeuence. It enhances the binding of Ribosome and mRNA.
+
This part is constructed to measure translation efficiency of SD8 (BBa_K1084104).
−
Also, it is said that the strength of the RBS, depends on the length of SD sequence.
+
We used LacZα (BBa_I732006) as reporter gene and performed β-Galactosidase assay.
  
  
  
 
===Source===
 
===Source===
 +
 
Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.
 
Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.
  
 
===References===
 
===References===
 
V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology
 
V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology

Latest revision as of 17:34, 27 September 2013

pTet SD8 LacZα dT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is constructed to measure translation efficiency of SD8 (BBa_K1084104). We used LacZα (BBa_I732006) as reporter gene and performed β-Galactosidase assay.


Source

Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.

References

V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology