Difference between revisions of "Part:BBa K1151004:Design"

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===Design Notes===
 
===Design Notes===
−
None
 
  
 +
This part was amplified by PCR from the plasmid received.
  
 +
                                  [[File:pexbBUnisalento.jpg]]
 +
 +
'''Figure 1:''' PCR results of pexbB.
 +
 +
 +
'''Primers used''' (including Biobricks' Prefix and Suffix, lowercase):
 +
 +
''pexbBFor:'' gtttcttcgaattcgcggccgcttctagagACTGGATTTAAATGGTTG
 +
 +
''pexbBRev:'' gtttcttcctgcagcggccgctactagtaGCACCCTATAAGAAGGCATC
  
 
===Source===
 
===Source===
  
−
This part derives from H. pylori genomic sequence, and it was amplified by PCR.
+
We received this part by Dr. Alberto Danielli, a researcher in molecular biology at the Department of Pharmacology and Biotechnology at the University of Bologna. He sent us the double divergent promoter (from the genome of ''H. pylori G27'') inserted into a pGEM T-Easy plasmid (as pNKTB).
  
 
===References===
 
===References===
  
 
1.  Delany et al,  In vitro analysis of protein-operator interactions of the NikR and Fur Metal-Responsive Regulators of coregulated genes in  Helicobacter pylori.  J Bacteriol. 2005 Nov;187(22):7703-15.
 
1.  Delany et al,  In vitro analysis of protein-operator interactions of the NikR and Fur Metal-Responsive Regulators of coregulated genes in  Helicobacter pylori.  J Bacteriol. 2005 Nov;187(22):7703-15.

Latest revision as of 11:12, 3 October 2013


pexbB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified by PCR from the plasmid received. 

                                 PexbBUnisalento.jpg

Figure 1: PCR results of pexbB.


Primers used (including Biobricks' Prefix and Suffix, lowercase):

pexbBFor: gtttcttcgaattcgcggccgcttctagagACTGGATTTAAATGGTTG

pexbBRev: gtttcttcctgcagcggccgctactagtaGCACCCTATAAGAAGGCATC

Source

We received this part by Dr. Alberto Danielli, a researcher in molecular biology at the Department of Pharmacology and Biotechnology at the University of Bologna. He sent us the double divergent promoter (from the genome of H. pylori G27) inserted into a pGEM T-Easy plasmid (as pNKTB).

References

1. Delany et al, In vitro analysis of protein-operator interactions of the NikR and Fur Metal-Responsive Regulators of coregulated genes in Helicobacter pylori. J Bacteriol. 2005 Nov;187(22):7703-15.