Difference between revisions of "Part:BBa K1139150"

Latest revision as of 21:48, 28 October 2013

Prm/lac-GFP-TT

PRM/lac is a hybrid promoter that is activated by lamda repressor (CI) and repressed by LacI repressor (Fig. 1).
On the downstream of the promoter, GFP is inserted as a reporter.

Fig. 1. Our newly designed hybrid promoter

To characterize the function of the RM/lac hybrid promoter (BBa_K1139151), we constructed this part PRM/lac-GFP (BBa_K1139150) by inserting RM/lac promoter upstream of a GFP coding sequence. By using reporter cells that contain PRM/lac-GFP, we measured the fluorescence intensity of the cells induced by CI and IPTG (Fig. 2).
We saw that our new RM/lac hybrid promoter was actually activated by CI through an induction assay (Fig. 3).

Fig. 2. Fluorescence intensity detected by flow cytometer

Fig. 3. Comparison of N99 and JM2.300

For more information, see [http://2013.igem.org/Team:Tokyo_Tech/Experiment/RM-lac_Hybrid_Promoter_Assay our work in Tokyo_Tech 2013 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 769

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1139150 short</partinfo>
-Prm/lac is a hybrid promoter that is modified to be activated by lamda repressor (CI) and repressed by LacI repressor.
+PRM/lac is a hybrid promoter that is activated by lamda repressor (CI) and repressed by LacI repressor (Fig. 1).<br>
-On the downstream of the promoter, ''GFP'' is inserted as a reporter.
+On the downstream of the promoter, GFP is inserted as a reporter.<br>
-[[Image:Titech2013_parts_K1139150_Fig1A.jpg|thumb|center|500px|'''Fig. 1-A.''' Our new designed hybrid promoter]]
+[[Image:titech2013_parts_K1139150_main_Fig1.jpg|thumb|center|250px|<b>Fig. 1.</b> Our newly designed hybrid promoter]]
-To characterize the function of the ''rm/lac'' hybrid promoter ([https://parts.igem.org/Part:BBa_K1139151 BBa_K1139151]), we constructed this part Prm/lac-''GFP'' ([https://parts.igem.org/Part:BBa_K113950 BBa_K113950]) by inserting ''rm/lac'' promoter in front of a GFP coding sequence. By using the reporter cell that contains Prm/lac-GFP, we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, CI and IPTG.  We confirmed that our new ''rm/lac'' hybrid promoter was actually activated by CI through an induction assay (Fig. 1-C).
+To characterize the function of the <i>RM/lac</i> hybrid promoter (<partinfo>BBa_K1139151</partinfo>), we constructed this part PRM/lac-GFP (<partinfo>BBa_K1139150</partinfo>) by inserting ''RM/lac'' promoter upstream of a GFP coding sequence. By using reporter cells that contain PRM/lac-GFP, we measured the fluorescence intensity of the cells induced by CI and IPTG (Fig. 2). <br>
+We saw that our new ''RM/lac'' hybrid promoter was actually activated by CI through an induction assay (Fig. 3).<br>
-[[Image:Titech2013_parts_K1139150_Fig1B.jpg|thumb|center|500px|'''Fig. 1B.''' Fluorescence intensity detected by flow cytometer]]
+[[Image:titech2013_parts_K1139150_main_Fig2.jpg|thumb|left|310px|<b>Fig. 2.</b> Fluorescence intensity detected by flow cytometer]]
-[[Image:Titech2013_parts_K1139150_Fig1C.jpg|thumb|center|500px|'''Fig. 1C.''' Comparison of N99 and JM2.300]]
+[[Image:titech2013_parts_K1139150_main_Fig3.jpg|thumb|none|240px|<b>Fig. 3.</b> Comparison of N99 and JM2.300]]
+<br>
+For more information, see [http://2013.igem.org/Team:Tokyo_Tech/Experiment/RM-lac_Hybrid_Promoter_Assay our work in Tokyo_Tech 2013 wiki].
 <!-- Add more about the biology of this part here