Difference between revisions of "Part:BBa K1140003:Design"
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===References=== | ===References=== | ||
| − | Elowitz | + | 1. Elowitz MB and Leibler S, (2000). A synthetic oscillatory network of transcriptional regulators, ''Nature'', 20;403(6767):335-8. |
| + | |||
| + | 2. Neupert J, Karcher D, Bock R, (2008) Design of simple synthetic RNA thermometers for temperature- controlled gene expression in ''Escherichia coli''. Nucleic Acids Res, 36(19):e124. | ||
Latest revision as of 03:47, 26 September 2013
pCons + 37 oC RNA thermometer + LacI (LVA)
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 44
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
prefix: EcoRI and XbaI sufix: SpeI and PstI.
Source
Obtained by de novo synthesis. The constitutive promoter corresponds to the sequence of part BBa_J23119, the most potent member of a family of promoter variants obtained from a combinatorial library. Transcription factor LacI is the same one found in E. coli lac operon and the sequence employed is the same as the one from part BBa_C0012, which is the same as the one employed in the Elowitz and Leibler 2000 study as stated in the “design” section of the registry entry for part BBa_C0012, this LacI variant differs from the wild-type in that the GTG start present in nature was changed to an ATG start.
References
1. Elowitz MB and Leibler S, (2000). A synthetic oscillatory network of transcriptional regulators, Nature, 20;403(6767):335-8.
2. Neupert J, Karcher D, Bock R, (2008) Design of simple synthetic RNA thermometers for temperature- controlled gene expression in Escherichia coli. Nucleic Acids Res, 36(19):e124.