Difference between revisions of "Part:BBa K1014000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | TAATAA double stop codon added. | + | <p></p>TAATAA double stop codon added. |
+ | <p></p>Codon optimization for E.coli was done using [http://www.jcat.de/ JCat] an online Codon Adaptation Tool which was published in NAR (Nucleic Acids Research). | ||
+ | <p></p>Codon optimization results was present on [http://2013.igem.org/Team:HIT-Harbin/Parts our wiki] | ||
===Source=== | ===Source=== | ||
− | + | <p></p>[http://www.ncbi.nlm.nih.gov/nuccore/NC_014500.1?report=genbank&from=2646451&to=2647434&strand=true GeneBank NC_014500.1] | |
− | + | <p></p>[https://parts.igem.org/wiki/index.php/Part:BBa_M39011 BBa_M39011] | |
===References=== | ===References=== | ||
− | Grote | + | |
+ | <p></p>[http://jb.asm.org/content/193/8/2076.short J. Bacteriol. 193 (8), 2076-2077 (2011)] | ||
+ | <p></p>[http://nar.oxfordjournals.org/content/33/suppl_2/W526.short Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host] |
Latest revision as of 06:06, 26 September 2013
HrpS Gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 157
Illegal AgeI site found at 163
Illegal AgeI site found at 298
Illegal AgeI site found at 456 - 1000COMPATIBLE WITH RFC[1000]