Difference between revisions of "Part:BBa K1031013"
 
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<partinfo>BBa_K1031013 short</partinfo>
 
<partinfo>BBa_K1031013 short</partinfo>
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<p>For detailed information concerning Band-pass Filter, please visit <a href="http://2013.igem.org/Team:Peking/Project/BandpassFilter">2013 Peking iGEM Band-pass Filter</a></p>
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<img src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg" style="width:960px;"/>
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== '''Components and Characterization''' ==
 
== '''Components and Characterization''' ==
  
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K1031013 consists of NahR[https://parts.igem.org/Part:BBa_J61051], Psal promoter and transcription factor CƖ. CƖ functions as a repressor of hybrid promoter[https://parts.igem.org/Part:BBa_K1031021]. It is the repression branch of the Band-pass Filter circuit ('''Fig 1''').
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K1031013 consists of NahR[https://parts.igem.org/Part:BBa_J61051], Psal promoter and transcription factor CƖ. CƖ functions as a repressor of <html><a href="https://parts.igem.org/Part:BBa_K1031021">hybrid promoter</a></html>. It is the repression node of the Band-pass Filter circuit ('''Fig 1''').
  
 
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/4/43/Peking2013_part_K1031013.png/800px-Peking2013_part_K1031013.png", width=700px; />
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/4/43/Peking2013_part_K1031013.png/800px-Peking2013_part_K1031013.png" style=" width:750px; margin-left:100px" />
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<p style="text-align:center"><b>Fig 1</b> Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating Psal promoter shown in light blue. Once pSal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibit hybrid promoter marked with two colors, thus inhibit expression of sfGFP. 
 
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'''Fig 1''' Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating Psal promoter shown in light blue. Once pSal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibit hybrid promoter marked with two colors, thus inhibit expression of sfGFP. 
 
 
   
 
   
  
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== '''Data shown''' ==
 
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Previous work done by Dr. Lou Chunbo showed CƖ had obvious inhibiting effect to downstream promoter. ('''Fig 2''').
 
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/3/35/Peking2013_part_C1.jpg/800px-Peking2013_part_C1.jpg", width=600px; />
 
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'''Fig 2''' Induction ratio of repression circuit when exposed to inducers. The concentration of inducer salicylic acid is fixed at 100µM. Horizontal axis represents IPTG at different concentration. Vertical axis stands for induction ratio calculated from fluorescence intensity. (Data from Dr.Lou Chunbo)
 
  
  

Latest revision as of 20:57, 27 September 2013

NahR-Psal-RBS-RiboJ-CI

For detailed information concerning Band-pass Filter, please visit 2013 Peking iGEM Band-pass Filter


Components and Characterization

K1031013 consists of NahR[1], Psal promoter and transcription factor CƖ. CƖ functions as a repressor of hybrid promoter. It is the repression node of the Band-pass Filter circuit (Fig 1).

Fig 1 Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating Psal promoter shown in light blue. Once pSal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibit hybrid promoter marked with two colors, thus inhibit expression of sfGFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]