Difference between revisions of "Part:BBa K1088014"

Latest revision as of 18:47, 28 October 2013

E. coli dxs (lac promoter with lac inhibitor: IPTG inducible)

This part consist of the dxs gene derived from E. coli under the control of the lac promoter and has a strong RBS.

To repress expression from the lac promoter, the lacI:LVA gene (under a constitutive promoter with a strong RBS and a efficient terminator) was placed upstream to the dxs gene. The repression can be relieved with addition of IPTG, which binds and inhibits the function of LacI:LVA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 2158

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1088014 short</partinfo>
-This part consist of the ''dxs'' gene derived from ''E. coli'' under the control of the lac promoter and has a strong RBS.
+This part consist of the ''dxs'' gene derived from ''E. coli'' under the control of the ''lac'' promoter and has a strong RBS.
-To repress expression from the lac promoter, the ''lacI:LVA'' gene (under a constitutive promoter, with a strong RBS and a efficient terminator) was placed counter-clockwise to the ''dxs'' gene. The repression can be relieved with addition of IPTG, which binds and inhibits the function of LacI:LVA.
+To repress expression from the ''lac'' promoter, the ''lacI:LVA'' gene (under a constitutive promoter with a strong RBS and a efficient terminator) was placed upstream to the ''dxs'' gene. The repression can be relieved with addition of IPTG, which binds and inhibits the function of LacI:LVA.
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