Difference between revisions of "Part:BBa K1031013"
 
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<partinfo>BBa_K1031013 short</partinfo>
 
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<p>For detailed information concerning Band-pass Filter, please visit <a href="http://2013.igem.org/Team:Peking/Project/BandpassFilter">2013 Peking iGEM Band-pass Filter</a></p>
  
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cI protein,which is a transcription factor,is a part of bandpass filter.It can repress the hybrid promoter K1031021[https://parts.igem.org/Part:BBa_K1031021].In order to fine-tune the band-pass filter,we construct a libiary of rbs before cI protein.
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== '''Components and Characterization''' ==
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===Usage and Biology===
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K1031013 consists of NahR[https://parts.igem.org/Part:BBa_J61051], Psal promoter and transcription factor CƖ. CƖ functions as a repressor of <html><a href="https://parts.igem.org/Part:BBa_K1031021">hybrid promoter</a></html>. It is the repression node of the Band-pass Filter circuit ('''Fig 1''').
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/4/43/Peking2013_part_K1031013.png/800px-Peking2013_part_K1031013.png" style=" width:750px; margin-left:100px" />
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<p style="text-align:center"><b>Fig 1</b> Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating Psal promoter shown in light blue. Once pSal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibit hybrid promoter marked with two colors, thus inhibit expression of sfGFP. 
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== '''Sequence and Features''' ==
 
== '''Sequence and Features''' ==
 
<partinfo>BBa_K1031013 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1031013 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1031013 parameters</partinfo>
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<partinfo>BBa_K1031011 parameters</partinfo>
 
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Latest revision as of 20:57, 27 September 2013

NahR-Psal-RBS-RiboJ-CI

For detailed information concerning Band-pass Filter, please visit 2013 Peking iGEM Band-pass Filter


Components and Characterization

K1031013 consists of NahR[1], Psal promoter and transcription factor CƖ. CƖ functions as a repressor of hybrid promoter. It is the repression node of the Band-pass Filter circuit (Fig 1).

Fig 1 Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating Psal promoter shown in light blue. Once pSal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibit hybrid promoter marked with two colors, thus inhibit expression of sfGFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]