Difference between revisions of "Part:BBa K1157020:Experience"

(Applications of BBa_K1157020)
(Applications of BBa_K1157020)
 
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===Applications of BBa_K1157020===
 
===Applications of BBa_K1157020===
We tested the function of Kii57020 by testing its GFP fluorescence expression. We used ELISA plate reader to read the fluorescence of this construct under different concentrations of C-4 AHLs. Our biosensor is transformed into competent cell ''E. Coli'' DH5α cultured overnight and then diluted until OD value=0.1. Then we read fluorescence expression from 0 to 4 hours.The results are as follows. [[File:Pc Rhl GFP.jpg]]
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Team NYMU_Taipei tested the function of Kii57020 by testing its GFP fluorescence expression. They used ELISA plate reader to read the fluorescence of this construct under different concentrations of C-4 AHLs. This biosensor is transformed into competent cell ''E. Coli'' DH5α cultured overnight and then diluted until OD value=0.1. Then we read fluorescence expression from 0 to 4 hours.The results are as follows. [[File:Pc Rhl GFP.jpg]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 11:37, 27 September 2013


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Applications of BBa_K1157020

Team NYMU_Taipei tested the function of Kii57020 by testing its GFP fluorescence expression. They used ELISA plate reader to read the fluorescence of this construct under different concentrations of C-4 AHLs. This biosensor is transformed into competent cell E. Coli DH5α cultured overnight and then diluted until OD value=0.1. Then we read fluorescence expression from 0 to 4 hours.The results are as follows. Pc Rhl GFP.jpg

User Reviews

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