Difference between revisions of "Part:BBa K1157020:Experience"

Latest revision as of 11:37, 27 September 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1157020

Team NYMU_Taipei tested the function of Kii57020 by testing its GFP fluorescence expression. They used ELISA plate reader to read the fluorescence of this construct under different concentrations of C-4 AHLs. This biosensor is transformed into competent cell E. Coli DH5α cultured overnight and then diluted until OD value=0.1. Then we read fluorescence expression from 0 to 4 hours.The results are as follows.

User Reviews

UNIQf89d8f80f5194c05-partinfo-00000000-QINU UNIQf89d8f80f5194c05-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K1157020===
-We tested the function of Kii57020 by testing its GFP fluorescence expression. We used ELISA plate reader to read the fluorescence of this construct under different concentrations of C-4 AHLs. Our biosensor is transformed into competent cell ''E. Coli'' DH5α cultured overnight and then diluted until OD value=0.1. Then we read fluorescence expression from 0 to 4 hours.The results are as follows. [[File:Pc Rhl GFP.jpg]]
+Team NYMU_Taipei tested the function of Kii57020 by testing its GFP fluorescence expression. They used ELISA plate reader to read the fluorescence of this construct under different concentrations of C-4 AHLs. This biosensor is transformed into competent cell ''E. Coli'' DH5α cultured overnight and then diluted until OD value=0.1. Then we read fluorescence expression from 0 to 4 hours.The results are as follows. [[File:Pc Rhl GFP.jpg]]
 ===User Reviews===