Difference between revisions of "Part:BBa K1031420"
 
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<partinfo>BBa_K1031420 short</partinfo>
 
<partinfo>BBa_K1031420 short</partinfo>
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<p>For detailed information concerning HcaR and Ph promoter, please visit <a href="http://2013.igem.org/Team:Peking/Project/BioSensors/HcaR">2013 Peking iGEM Biosensor HcaR</a></p>
  
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<img src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg" style="width:960px;"/>
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== '''Structure''' ==
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<img src="https://static.igem.org/mediawiki/igem.org/4/41/Peking2013_HcaRFig1.jpg" style="width:600px; margin-left:170px" />
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<p style="text-align:center"><b>Fig 1</b> Structure of <i>Ph</i> promoter regulated by HcaR and chemical pathway.
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For the biosensor circuit Ph/J23114-HcaR, we created a library of RBS (Ribosome Binding Site) including B0031, B0032 and B0034 for tuning the expression level of reporter gene sfGFP.  
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For the biosensor circuit ''Ph''/HcaR, we created a library of RBS (Ribosome Binding Site) including B0031, B0032 and B0034 for tuning the expression level of reporter gene sfGFP.  
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K1031420 is composed of three elements, Pc promoter activated by HcaR, RBS B0031[https://parts.igem.org/Part:BBa_B0031] and reporter gene sfGFP ('''Fig 1a'''). Induction ratio was calculated from fluorescence intensity, showing the performance of K1031420('''Fig 1b''')
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K1031420 is composed of three elements, ''Ph'' promoter activated by HcaR, RBS <html><a href="https://parts.igem.org/Part:BBa_B0031">B0031</a></html> and reporter gene sfGFP ('''Fig 1a'''). Induction ratio was tested when K1031420 is in collocation with J23106-HcaR and J23114-HcaR, as shown in the dashed boxes ('''Fig 1b''')
  
 
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/2/22/Peking2013_part_RBS_library_31_HcaR.png/618px-Peking2013_part_RBS_library_31_HcaR.png", width=400px; />
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/1/1c/Peking2013_part_K1031420.png/800px-Peking2013_part_K1031420.png", width=950px; />
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<p style="text-align:center"><b>Fig 2</b> Construction and tunning of reporter circuit <i>Ph</i>-B0031-sfGFP. (<b>a</b>) The orange arrow represents <i>Ph</i> promoter activated by HcaR. The green oval stands for RBS B0031. sfGFP coding sequence is shown with dark blue, while terminator B0015 is in dark red. (<b>b</b>) Horizontal axis represents biosensor circuit adopting different combination of <i>Pc</i> promoter and RBS. Vertical axis stands for induction ratio. The dashed box refers to data for reporter circuit <i>Pc</i>-B0031-sfGFP in collocation with biosensor circuits J23106-HcaR and J23114-HcaR respectively.
 
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'''Fig 1''' Construction and tunning of reporter circuit Pc-B0031-sfGFP ('''a''') The orange arrow represents ''Pc'' promoter for HbpR. The green oval stands for RBS B0031[https://parts.igem.org/Part:BBa_B0031]. sfGFP coding sequence is shown with dark blue, while terminator B0015[https://parts.igem.org/Part:BBa_B0015] is in dark red. ('''b''') Horizontal axis represents biosensor circuit adopting different combination of ''Pc'' promoter and RBS. Vertical axis stands for induction ratio. The dashed box refers to data for reporter circuit ''Pc''-B0031-sfGFP in collocation with biosensor circuit J23114-HcaR.
 
  
  

Latest revision as of 22:18, 27 September 2013

Ph-B0031-sfGFP-Terminator (HcaR)

For detailed information concerning HcaR and Ph promoter, please visit 2013 Peking iGEM Biosensor HcaR


Structure

Fig 1 Structure of Ph promoter regulated by HcaR and chemical pathway.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 113


Construction and data

For the biosensor circuit Ph/HcaR, we created a library of RBS (Ribosome Binding Site) including B0031, B0032 and B0034 for tuning the expression level of reporter gene sfGFP. K1031420 is composed of three elements, Ph promoter activated by HcaR, RBS B0031 and reporter gene sfGFP (Fig 1a). Induction ratio was tested when K1031420 is in collocation with J23106-HcaR and J23114-HcaR, as shown in the dashed boxes (Fig 1b)

Fig 2 Construction and tunning of reporter circuit Ph-B0031-sfGFP. (a) The orange arrow represents Ph promoter activated by HcaR. The green oval stands for RBS B0031. sfGFP coding sequence is shown with dark blue, while terminator B0015 is in dark red. (b) Horizontal axis represents biosensor circuit adopting different combination of Pc promoter and RBS. Vertical axis stands for induction ratio. The dashed box refers to data for reporter circuit Pc-B0031-sfGFP in collocation with biosensor circuits J23106-HcaR and J23114-HcaR respectively.