Difference between revisions of "Part:BBa K1051151"
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<partinfo>BBa_K1051151 short</partinfo> | <partinfo>BBa_K1051151 short</partinfo> | ||
| − | + | GalI+ZRC1+GFP+TYB | |
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<partinfo>BBa_K1051151 parameters</partinfo> | <partinfo>BBa_K1051151 parameters</partinfo> | ||
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| + | ===principle=== | ||
| + | <p>Though it accounts a small ratio in the cell space, mitochondria possess about 10% to 15% proteins encoded by nuclear genes in eukaryotic organisms. These proteins are synthesized in cytosol and then recognized by the membrane receptors of mitochondria. Translocases in the outer and inner membrane of mitochondria mediate the import and intra-mitochondrial sorting of these proteins. ATP is used as an energy source; Chaperones and auxiliary factors assist in folding and assembly of mitochondrial proteins into their native, three-dimensional structures. As shown in the figure above, beta-barrel outer-membrane proteins (dark green), precursor proteins (brown) with positively charged amino-terminal presequences and multispanning inner-membrane proteins (blue) with internal targeting signals are recognized by specific receptors of the outer mitochondrial membrane (TOM) translocases Tom20, Tom22 and/or Tom70. The precursor proteins are then translocated through a small Tom proteins of the TOM complex, Tom40 pore, which the TOM complex contains two or three.</p> | ||
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| + | https://static.igem.org/mediawiki/2013/1/1f/Figure1.protein-import_pathways_for_mitochondrial_proteins.png | ||
| + | Fig. mit pathway | ||
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| + | ===Result=== | ||
Latest revision as of 13:16, 23 October 2013
GalI+ZRC1+GFP+TYB
GalI+ZRC1+GFP+TYB
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 607
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 610
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 607
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 607
Illegal AgeI site found at 150 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1601
principle
Though it accounts a small ratio in the cell space, mitochondria possess about 10% to 15% proteins encoded by nuclear genes in eukaryotic organisms. These proteins are synthesized in cytosol and then recognized by the membrane receptors of mitochondria. Translocases in the outer and inner membrane of mitochondria mediate the import and intra-mitochondrial sorting of these proteins. ATP is used as an energy source; Chaperones and auxiliary factors assist in folding and assembly of mitochondrial proteins into their native, three-dimensional structures. As shown in the figure above, beta-barrel outer-membrane proteins (dark green), precursor proteins (brown) with positively charged amino-terminal presequences and multispanning inner-membrane proteins (blue) with internal targeting signals are recognized by specific receptors of the outer mitochondrial membrane (TOM) translocases Tom20, Tom22 and/or Tom70. The precursor proteins are then translocated through a small Tom proteins of the TOM complex, Tom40 pore, which the TOM complex contains two or three.
Fig. mit pathway