Difference between revisions of "Part:BBa J37016:Design"

 
(→‎Design Notes)
 
(2 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J37016 short</partinfo>
 
<partinfo>BBa_J37016 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
na
 
  
 +
*The part is designed to produce both LuxR and GFP in response to different AHL concentrations.
 +
with LuxI under the control of Lux pR *LuxR and GFP are under the control of the promoter luxpR, which is activated by the heterodimer AHL/LuxR
 +
*The part has been ligated from the following previous parts: <bbpart>BBa_J37019</bbpart>,
 +
<bbpart>J37025</bbpart>*Expression of GFP downstream of the promoter serves as an indicator of the production of LuxR.
  
 +
*More details about design and motivation behind the part can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Predator_Construct/Design here].
  
 
===Source===
 
===Source===

Latest revision as of 15:14, 30 October 2006

Predator Molecule Generator Sensing Test (GFP output)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1532


Design Notes

  • The part is designed to produce both LuxR and GFP in response to different AHL concentrations.

with LuxI under the control of Lux pR *LuxR and GFP are under the control of the promoter luxpR, which is activated by the heterodimer AHL/LuxR

  • The part has been ligated from the following previous parts: BBa_J37019,

J37025*Expression of GFP downstream of the promoter serves as an indicator of the production of LuxR.

  • More details about design and motivation behind the part can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Predator_Construct/Design here].

Source

na

References