Difference between revisions of "Part:BBa K1045016"

Latest revision as of 14:12, 19 October 2013

n/a	Terminator BBa_B0015 with inversed Pre- and Suffix- DarR ORF with inversed Pre- and Suffix

Terminator BBa_B0015 with inversed Pre- and Suffix- DarR ORF with inversed Pre- and Suffix

This composite part is composed of BBa_K1045001, an inverse DarR coding sequence, fused to an inverse terminator, BBa_K1045009. DarR is a transcriptional repressor binding to the DarR operator sequence. Repression by DarR is enhanced by c-di-AMP. In E. coli, DarR seemed to act as a strong repressor even without c-di-AMP ([http://2013.igem.org/Team:Goettingen iGEM Göttingen 2013]). BBa_K1045016 could be cloned downstream of inverse promoter-RBS constructs (compare BBa_K1045010 (inverse RBS) and BBa_K1045011 (inverse promoter)) allowing it to be expressed (for example, see BBa_K1045017).

Secondary Structure

File:Mfold-K1045016-1.png

Measurement

[http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

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 <partinfo>BBa_K1045016 short</partinfo>
-* This composite part is composed of part BBa_K1045001, an inverse DarR coding sequence, fused to an inverse terminator, BBa_K1045009. DarR is a transcriptional repressor binding to the DarR operator sequence. Repression by DarR is enhanced by c-di-AMP. In ''E. coli'', DarR seemed to act as a quite strong repressor even without c-di-AMP (Team Göttingen 2013). Part BBa_K1045016 could be cloned downstream of inverse promoter-RBS constructs (compare parts BBa_K1045010 (inverse RBS) and BBa_K1045011 (inverse promoter)) allowing it to be expressed (for example, see part BBa_K1045017).
+* This composite part is composed of [[Part:BBa_K1045001|BBa_K1045001]], an inverse DarR coding sequence, fused to an inverse terminator, [[Part:BBa_K1045009|BBa_K1045009]]. DarR is a transcriptional repressor binding to the DarR operator sequence. Repression by DarR is enhanced by c-di-AMP. In ''E. coli'', DarR seemed to act as a strong repressor even without c-di-AMP ([http://2013.igem.org/Team:Goettingen iGEM Göttingen 2013]). [[Part:BBa_K1045016|BBa_K1045016]] could be cloned downstream of inverse promoter-RBS constructs (compare [[Part:BBa_K1045010|BBa_K1045010]] (inverse RBS) and [[Part:BBa_K1045011|BBa_K1045011]] (inverse promoter)) allowing it to be expressed (for example, see [[Part:BBa_K1045017|BBa_K1045017]]).
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