Difference between revisions of "Part:BBa K1045010"
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<partinfo>BBa_K1045010 short</partinfo> | <partinfo>BBa_K1045010 short</partinfo> | ||
| − | This part encodes an inverted RBS based on part BBa_B0034, a strong RBS. The prefix sequence is placed at the end of the RBS sequence. The suffix is located at its beginning. It might allow the translation of mRNAs of inverted expression units (for an example, see BBa_K1045017). An inverted RBS might be helpful if you want to clone two genes into one vector so that both genes are transcribed into diverging directions. | + | This part encodes an inverted (reverse complement) RBS based on part [[Part:BBa_B0034|BBa_B0034]], a strong RBS. The prefix sequence is placed at the end of the RBS sequence. The suffix is located at its beginning. It might allow the translation of mRNAs of inverted expression units (for an example, see [[Part:BBa_K1045017|BBa_K1045017]]). An inverted RBS might be helpful if you want to clone two genes into one vector so that both genes are transcribed into diverging directions. |
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Latest revision as of 03:01, 29 October 2017
RBS BBa_B0034 with inversed Pre- and Suffix
This part encodes an inverted (reverse complement) RBS based on part BBa_B0034, a strong RBS. The prefix sequence is placed at the end of the RBS sequence. The suffix is located at its beginning. It might allow the translation of mRNAs of inverted expression units (for an example, see BBa_K1045017). An inverted RBS might be helpful if you want to clone two genes into one vector so that both genes are transcribed into diverging directions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]