Difference between revisions of "Part:BBa K1060012"

Latest revision as of 09:47, 30 September 2013

Methyl Salicylate Production under TetR regulation

KU_Leuven 2013 requested the BBa_J45700 brick from MIT 2006 and recloned into the pSB1C3 standard plasmid backbone (BBa_K1060003). In this part we removed the lac promoter in front of the pchBA gene to get a constitutive expression of all genes needed for methyl salicylate production in the absence of the TetR repressor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 746
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1656
Illegal NgoMIV site found at 2060
Illegal NgoMIV site found at 2185
Illegal NgoMIV site found at 2196
Illegal NgoMIV site found at 2477
Illegal NgoMIV site found at 2945
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 287

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1060012 short</partinfo>
-KU_Leuven 2013 requested the BBa_J45700 brick from MIT 2006 and recloned into the pSB1C3 standard plasmid backbone (BBa_K1060003). In this part we removed the lac promoter in front of the pchBA gene to get a constitutive expression of all genes needed for methyl salicylate production. We did this by amplifying the For the assembly of this part we refer to the original MIT brick BBa_J45700 => "We assembled a complete wintergreen odor biosynthetic system (BBa_J45700) by combining the salicylic acid (BBa_J45320) and wintergreen odor generators (BBa_J45120). BBa_J45700 catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor."
+KU_Leuven 2013 requested the <partinfo>BBa_J45700</partinfo> brick from MIT 2006 and recloned into the pSB1C3 standard plasmid backbone (<partinfo>BBa_K1060003</partinfo>). In this part we removed the ''lac'' promoter in front of the ''pchBA'' gene to get a constitutive expression of all genes needed for methyl salicylate production in the absence of the TetR repressor.