Difference between revisions of "Part:BBa K1067002"

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<partinfo>BBa_K1067002 short</partinfo>
 
<partinfo>BBa_K1067002 short</partinfo>
  
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Cytochrome A (c-554) and cytochrome X (Probable tetraheme cytochrome c-type) both from ''Nitrosomonas europaea''.
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Cytochrome A and cytochrome X ([http://ecocyc.org/NEUR228410/NEW-IMAGE?type=LOCUS-POSITION&object=GJNO-2382&chromosome=CHROMOSOME-1 link to Biocyc]) both from ''Nitrosomonas europaea''.
  
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The two cytochromes are involved in the process of transferring electrons from [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1067004 HAO] to [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1067003 AMO] in the following reactions:
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===Usage and Biology===
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The two cytochromes are involved in the process of transferring electrons from HAO to AMO in the following reactions:
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HAO reaction, 2 electrons gained:
 
HAO reaction, 2 electrons gained:
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NH<sub>2</sub>OH + H<sub>2</sub>O + 2 cycAX<sub>ox</sub> &rarr; NO<sub>2</sub><sup>-</sup> + 2 cycAX<sub>red</sub> + 5 H<sup>+</sup>
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'''NH<sub>2</sub>OH + H<sub>2</sub>O + 2 cycAX<sub>ox</sub> &rarr; NO<sub>2</sub><sup>-</sup> + 2 cycAX<sub>red</sub> + 5 H<sup>+</sup>'''
  
 
AMO reaction 2 electrons used:
 
AMO reaction 2 electrons used:
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NH<sub>3</sub> + O<sub>2</sub> + cycAX<sub>red</sub> &rarr; NH<sub>2</sub>OH + NO<sub>2</sub>O + cycAX<sub>ox</sub>
 
  
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'''NH<sub>3</sub> + O<sub>2</sub> + cycAX<sub>red</sub> + 2 H<sup>+</sup> &rarr; NH<sub>2</sub>OH + H<sub>2</sub>O + cycAX<sub>ox</sub>'''
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===Usage and Biology===
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When highly expressed in ''E.coli'' the cells turn dark red. If grown on media with supplementation of d-aminolevulinic acid (ALA) it tends to get a darker color because of the extra heme incorporated. 
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https://parts.igem.org/Part:BBa_K2272019
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1067002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1067002 SequenceAndFeatures</partinfo>

Latest revision as of 02:21, 28 October 2017

Cytochrome A and X from Nitrosomonas europaea

Cytochrome A and cytochrome X ([http://ecocyc.org/NEUR228410/NEW-IMAGE?type=LOCUS-POSITION&object=GJNO-2382&chromosome=CHROMOSOME-1 link to Biocyc]) both from Nitrosomonas europaea.

The two cytochromes are involved in the process of transferring electrons from HAO to AMO in the following reactions:

HAO reaction, 2 electrons gained:

NH2OH + H2O + 2 cycAXox → NO2- + 2 cycAXred + 5 H+

AMO reaction 2 electrons used:

NH3 + O2 + cycAXred + 2 H+ → NH2OH + H2O + cycAXox


Usage and Biology

When highly expressed in E.coli the cells turn dark red. If grown on media with supplementation of d-aminolevulinic acid (ALA) it tends to get a darker color because of the extra heme incorporated.

https://parts.igem.org/Part:BBa_K2272019 Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal PstI site found at 51
    Illegal PstI site found at 490
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal PstI site found at 51
    Illegal PstI site found at 490
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal BglII site found at 448
    Illegal BamHI site found at 235
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal PstI site found at 51
    Illegal PstI site found at 490
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal PstI site found at 51
    Illegal PstI site found at 490
    Illegal NgoMIV site found at 1140
  • 1000
    COMPATIBLE WITH RFC[1000]