Difference between revisions of "Part:BBa J25500"

Latest revision as of 02:28, 11 August 2006

Light-repressed mCherry (uses photosensor)

The photosensor BBa_M30109Natalie Kuldell used in her BE.109 course hooks into an OmpR-regulated mCherry+lva, BBa_J25010. This is an intermediate composite part intended to test the viability of our construction of the photosensor. Eventually we would like to control bioluminesence of the Lux system (LuxCDABE) with the photosensor.

Usage and Biology

I'm concerned that mCherry may only work in Yeast. Investigate when refining this design.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1702
Illegal BglII site found at 4190
Illegal BamHI site found at 65
Illegal XhoI site found at 2297
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1255
1000
COMPATIBLE WITH RFC[1000]

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 The photosensor <partinfo>M30109</partinfo>Natalie Kuldell used in her BE.109 course hooks into an OmpR-regulated mCherry+lva, <partinfo>J25010</partinfo>. This is an intermediate composite part intended to test the viability of our construction of the photosensor.  Eventually we would like to control bioluminesence of the Lux system (LuxCDABE) with the photosensor.
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
+I'm concerned that mCherry may only work in Yeast.  Investigate when refining this design.
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