|
|
| (30 intermediate revisions by 4 users not shown) |
| Line 1: |
Line 1: |
| − | __NOTOC__
| + | #REDIRECT [[Help:An Introduction to BioBricks]] |
| − | {|
| + | |
| − | |[https://parts.igem.org/wiki/index.php?title=Help:BioBrick_Prefix_and_Suffix https://static.igem.org/mediawiki/parts/d/d7/Partinplasmid.png]
| + | |
| − | |width=600px|
| + | |
| − | BioBricks are a set of interchangable parts, designed with a view to building biological systems in living cells. The picture to the left shows BioBrick part [[Part:BBa_B0034|BBa_0034]] within a [[Help:Plasmids|plasmid]]. The brick is flanked by a standard biobrick [[Help:BioBrick Prefix and Suffix|prefix (P) and suffix (S)]]. The yellow arrow represents the plasmid's antibiotic resistance gene and the green arrow, its origin of replication. Click the image to find out more or alternatively see:
| + | |
| − | | + | |
| − | *[[Part Names|BioBrick Names]]
| + | |
| − | *[[Part Types|Types of BioBrick]]
| + | |
| − | *[[Help:Plasmids/Construction Plasmids|Plasmids Introduction]]
| + | |
| − | |}
| + | |
| − | | + | |
| − | | + | |
| − | ==Building BioBrick Systems==
| + | |
| − | {|
| + | |
| − | |[[image:MultiPartsinplasmid.png]]
| + | |
| − | |width=600px|
| + | |
| − | BioBrick parts can be assembled to form useful devices, through a process often refeed to as 'Standard Assembly' This uses normal cloning techniques based on [[Assembly:Restriction enzymes|restriction enzymes]], purification, ligation, and transformation. BioBrick parts are [[Assembly:Composable|composable]]; allowing endless numbers of biobricks to be pieced together to form higher systems.
| + | |
| − | | + | |
| − | The assembly to the left shows 3 BioBricks assembled to create a simple device. You can learn more about this device by following the link to its associated page in the registry. You may like look into:
| + | |
| − | *[[Assembly:Standard assembly|Standard Assembly]] - <i>The classical method of BioBrick assembly, done at the lab bench</i>
| + | |
| − | *[[Assembly:Restriction enzymes|BioBrick Restriction Enzymes]]
| + | |
| − | *[[Assembly:Rolling assembly|Rolling Assembly]]
| + | |
| − | *[[Assembly:Robotic assembly| Automated assembly]] - <i>currently in late stage development at iGEM labs</i>
| + | |
| − | |}
| + | |
| − | | + | |
| − | ==Designing BioBrick Devices & Systems: Tools & Methods==
| + | |
| − | {|{{Click
| + | |
| − | |[[image:Buttonaddpart.gif]] | link=Add a Part to the Registry|width=75px | height=75px }}
| + | |
| − | | valign='middle' |
| + | |
| − | [https://parts.igem.org/cgi/partsdb/search.cgi https://static.igem.org/mediawiki/parts/8/85/Button3partsearch.gif]
| + | |
| − | |valign='top' |[https://parts.igem.org/cgi/assembly/blast.cgi https://static.igem.org/mediawiki/parts/4/48/Buttonsequenceanalysis.gif]
| + | |
| − | |width=600px|
| + | |
| − | BioBrick parts can be assembled to form useful devices, through a process often refeed to as 'Standard Assembly' This uses normal cloning techniques based on [[Assembly:Restriction enzymes|restriction enzymes]], purification, ligation, and transformation. BioBrick parts are [[Assembly:Composable|composable]]; allowing endless numbers of biobricks to be pieced together to form higher systems.
| + | |
| − | | + | |
| − | The assembly to the left shows 3 BioBricks assembled to create a simple device. You can learn more about this device by following the link to its associated page in the registry. You may like look into:
| + | |
| − | *[[Assembly:Standard assembly|Standard Assembly]] - <i>The classical method of BioBrick assembly, done at the lab bench</i>
| + | |
| − | *[[Assembly:Restriction enzymes|BioBrick Restriction Enzymes]]
| + | |
| − | *[[Assembly:Rolling assembly|Rolling Assembly]]
| + | |
| − | *[[Assembly:Robotic assembly| Automated assembly]] - <i>currently in late stage development at iGEM labs</i>
| + | |
| − | |}
| + | |
| − | | + | |
| − | ====Adding the part to the registry====
| + | |
| − | This protocol is quick and easy. A comprehensive guide can be found [[Basic Biobrick, example 1|here]]
| + | |
