Difference between revisions of "Part:BBa K1045012:Design"
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===Design Notes===
 
===Design Notes===
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This part was constructed using a hybridization oligo composed of BBa_J23110, followed by an XbaI/SpeI scar and the DarR operator sequence.
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This part was constructed using a hybridization oligo composed of [[Part:BBa_J23110|BBa_J23110]], followed by an ''XbaI''/''SpeI'' scar and the DarR operator sequence.
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===Source===
 
===Source===
  
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To be continued
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The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence originated from the Parts Registry page of [[Part:BBa_J23110|BBa_J23110]] and the sequence of the DarR operator found upstream of ''darR'' (Zhang ''et al''., 2013).
  
 
===References===
 
===References===
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Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in ''Mycobacterium smegmatis''”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096

Latest revision as of 19:01, 4 October 2013

Promoter with DarR operator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was constructed using a hybridization oligo composed of BBa_J23110, followed by an XbaI/SpeI scar and the DarR operator sequence.

Source

The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence originated from the Parts Registry page of BBa_J23110 and the sequence of the DarR operator found upstream of darR (Zhang et al., 2013).

References

Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096