Difference between revisions of "Part:BBa K1073003"
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<partinfo>BBa_K1073003 short</partinfo>
 
<partinfo>BBa_K1073003 short</partinfo>
  
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This construct is derived from BBa_K091117 and BBa_B0032. It combines the inducible promoter of th las Quorum sensing system of ''P. aeruginosa'' whitch is activated by LasR in combination with N-oxododecanoyl homoserine lactone (N-oxododecanoyl-HSL) with an RBS.
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This construct is derived from <partinfo>BBa_K091117</partinfo> and <partinfo>BBa_B0032</partinfo>. It combines the inducible promoter of the las Quorum sensing system of ''Pseudomonas aeruginosa'' with a RBS.
  
  
 
===Usage and Biology===
 
===Usage and Biology===
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The inducible Promotor from ''Pseudomonas aeruginosa'' is activated by LasR complexed with the specific autoinducer N-oxododecanoyl-HSL produced by LasI. The induction of the promoter  
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The inducible promoter from ''Pseudomonas aeruginosa'' is activated by LasR complexed with the specific autoinducer N-3-oxododecanoyl-HSL synthesized by LasI. The induction of the promoter  
 
in ''P. aeruginosa'' leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003].  
 
in ''P. aeruginosa'' leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003].  
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The device can be cloned in front of a desired gene of interest in order to induce its Expression by N-oxododecanoyl-HSL.
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The device can be cloned in front of a desired gene of interest in order to induce its expression by N-3-oxododecanoyl-HSL.
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For further Information see BBa_R0079.
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For further Information see <partinfo>BBa_R0079</partinfo>.
  
 
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Latest revision as of 15:42, 29 September 2013

Inducible promoter of the las quorum sensing system with RBS

This construct is derived from BBa_K091117 and BBa_B0032. It combines the inducible promoter of the las Quorum sensing system of Pseudomonas aeruginosa with a RBS.


Usage and Biology

The inducible promoter from Pseudomonas aeruginosa is activated by LasR complexed with the specific autoinducer N-3-oxododecanoyl-HSL synthesized by LasI. The induction of the promoter in P. aeruginosa leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003]. The device can be cloned in front of a desired gene of interest in order to induce its expression by N-3-oxododecanoyl-HSL. For further Information see BBa_R0079.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]