Difference between revisions of "Part:BBa K1045010:Design"

(→‎Design Notes)
(→‎Design Notes)
 
(5 intermediate revisions by 2 users not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
For cloning of RBS_BBa_B0034rev, hybridization oligos were used. Prefix and Suffix were switched to yield an inverted RBS.
+
For cloning of the inverse RBS '''BBa_K1045010''', hybridization oligos for the RBS [[Part:BBa_B0034|BBa_B0034]] were used. In these hybridization oligos, Prefix and Suffix were switched to yield an inverted RBS.
  
 
===Source===
 
===Source===
  
−
To be continued
+
The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence originated from the Parts Registry page of [[Part:BBa_B0034|BBa_B0034]].
  
 
===References===
 
===References===

Latest revision as of 16:47, 26 October 2013


RBS BBa_B0034 with inversed Pre- and Suffix


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For cloning of the inverse RBS BBa_K1045010, hybridization oligos for the RBS BBa_B0034 were used. In these hybridization oligos, Prefix and Suffix were switched to yield an inverted RBS.

Source

The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence originated from the Parts Registry page of BBa_B0034.

References