Difference between revisions of "Part:BBa K1157015:Experience"

Latest revision as of 10:00, 23 September 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1157015

We tested this circuit by transforming it in E. Coli DH5 alfa. We cultured the E. Coli in LB medium overnight, dilute until OD value=0.1, then added into ELISA plate reader for testing.(See testing protocol in NTU_Taida igem WIKI for details.) We tested different concentration of C-4 AHL to see whether the constitutive production of fluorescent protein will be inhibited through the CI-pCI circuit. The concentration unit is M. This is our functional results of testing BBa_K1157015 for 4 hours.

User Reviews

UNIQbe90f56876195259-partinfo-00000000-QINU UNIQbe90f56876195259-partinfo-00000001-QINU

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 ===Applications of BBa_K1157015===
-We tested this circuit by transforming it in ''E. Coli'' DH5 alfa. We cultured the ''E. Coli'' in LB medium overnight, dilute until OD value=0.1, then added into ELISA plate reader for testing.(See testing protocol in NTU_Taida igem WIKI for details.) This is our functional results of testing BBa_K1157015 for 4 hours. [[File:pC_pRhl_pCI]]
+We tested this circuit by transforming it in ''E. Coli'' DH5 alfa. We cultured the ''E. Coli'' in LB medium overnight, dilute until OD value=0.1, then added into ELISA plate reader for testing.(See testing protocol in NTU_Taida igem WIKI for details.) We tested different concentration of C-4 AHL to see whether the constitutive production of fluorescent protein will be inhibited through the CI-pCI circuit. The concentration unit is M. This is our functional results of testing BBa_K1157015 for 4 hours. [[File:pC_pRhl_pCI.jpg]]
 ===User Reviews===