Difference between revisions of "Part:BBa K1157015:Experience"
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===Applications of BBa_K1157015=== | ===Applications of BBa_K1157015=== | ||
| − | We tested this circuit by transforming it in ''E. Coli'' DH5 alfa. We cultured the ''E. Coli'' in LB medium overnight, dilute until OD value=0.1, then added into ELISA plate reader for testing.(See testing protocol in NTU_Taida igem WIKI for details.) This is our functional results of testing | + | We tested this circuit by transforming it in ''E. Coli'' DH5 alfa. We cultured the ''E. Coli'' in LB medium overnight, dilute until OD value=0.1, then added into ELISA plate reader for testing.(See testing protocol in NTU_Taida igem WIKI for details.) We tested different concentration of C-4 AHL to see whether the constitutive production of fluorescent protein will be inhibited through the CI-pCI circuit. The concentration unit is M. This is our functional results of testing BBa_K1157015 for 4 hours. [[File:pC_pRhl_pCI.jpg]] |
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 10:00, 23 September 2013
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1157015
We tested this circuit by transforming it in E. Coli DH5 alfa. We cultured the E. Coli in LB medium overnight, dilute until OD value=0.1, then added into ELISA plate reader for testing.(See testing protocol in NTU_Taida igem WIKI for details.) We tested different concentration of C-4 AHL to see whether the constitutive production of fluorescent protein will be inhibited through the CI-pCI circuit. The concentration unit is M. This is our functional results of testing BBa_K1157015 for 4 hours. 
User Reviews
UNIQ5c9fc5661f565c65-partinfo-00000000-QINU UNIQ5c9fc5661f565c65-partinfo-00000001-QINU