Difference between revisions of "Part:BBa E1010:Experinece"

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As part of the 2013 CU Boulder project we worked on separating RFP from AmilCP and during that process we ran into a problem that the fluorescence of RFP is too close to the absorption of AmilCP to tell them apart. What we ended up doing was measuring the spectrum of RFP from 400nm to 600nm.
 
  
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[[File:Cu_RFP.png|200px]]
 
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What we found is that RFP has a secondary absorbance peak at 502nm which is well clear of AmilCP. Under the devices we had access to this secondary peak also remained in the linear region of our device over a much wider concentration range.
 

Latest revision as of 23:54, 22 September 2013