Difference between revisions of "Help:Tag"

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[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Tag [Degradation] Tags] are genetic additions to the end of a sequence which mark a protein for degradation by various proteases within the cell.  This effectively decreases the protein's half life<br>
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#redirect [[Help:Proteins]]
One of the useful aspects of genetic tags is the ability to detect gene activity in a time-sensitive manner.
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Tags are often found on [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=reporter Reporter parts] and other types of [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Coding Protein Coding parts].<br>
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=="LVA, AAV, ASV, LAA" Tags==
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These are amino acid sequences which can be added to the C-terminal end of a protein.  These sequences mark the protein for immediate degradation by intracellular proteases which specifically target the C-terminus tail of proteins. 
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references: <br>
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# Keiler, KC et al.  "Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA."  1996. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8584937 Pubmed]
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# Andersen, JB et al.  "New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria".  1998.  [http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=9603842 link]
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==Future Plans==
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Our future plans for tags involve building proteins using biobricks.  To read more on this project, visit [https://dspace.mit.edu/handle/1721.1/32535 Ira Phillip's work on biobrick modification].
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Latest revision as of 21:40, 15 June 2017

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