Difference between revisions of "Part:BBa K1163101"

 
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1163101 short</partinfo>
 
<partinfo>BBa_K1163101 short</partinfo>
  
−
Promoter from E. coli that controls the AceB gene, involved in iron uptake and iron sensitive.
+
<br>
 +
<b>iGEM Evry 2013</b>
 +
 
 +
<p>
 +
<b>Description:</b>
 +
<br>
 +
The AceB gene encodes the malate synthase A enzyme, which is involved in carbon source management. Its promoter region has been extracted from the genome of Escherichia coli. We found after genomic research that the gene is under the regulation of fur, thus meaning it is sensitive to iron concentration variations.<br>
 +
<br>
 +
FUR (Ferric Uptake Regulation) is a <b>transcriptional repressor of genes involved in iron homeostasis</b>. In the presence of iron, FUR binds iron and dimerizes. This modification of conformation allows the linkage to a Fur Binding Site and inhibits the mRNA transcription of the downstream gene.
 +
<br>
 +
This 300 bp long promoter sequence contains a RBS and a FUR binding site, although it has not been clearly possible to identify them.
 +
</p>
 +
 
 +
<br>
  
−
FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription.
+
<p>
 +
<b>Characterization:</b>
 +
<br>
 +
We characterized its inhibition ability by cloning a superfolder GFP downstream at a iron concentration range from 10<sup>-7</sup> to 10<sup>-4</sup> mol.L<sup>-1</sup>.
 +
</p>
  
−
Why would you use this part?
+
<br>
  
−
This promoter allows you to have an iron-sensitive behaviour. In fact, in the presence of iron, the Fes promoter region decreases the expression of the downstream gene.  
+
<p>
 +
<b>Why would you use this part?</b>
 +
<br>
 +
This part should be used for iron sensing purposes in Escherichia coli.
 +
</p>
 +
<br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:44, 4 October 2013

pAceB promoter region


iGEM Evry 2013

Description:
The AceB gene encodes the malate synthase A enzyme, which is involved in carbon source management. Its promoter region has been extracted from the genome of Escherichia coli. We found after genomic research that the gene is under the regulation of fur, thus meaning it is sensitive to iron concentration variations.

FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In the presence of iron, FUR binds iron and dimerizes. This modification of conformation allows the linkage to a Fur Binding Site and inhibits the mRNA transcription of the downstream gene.
This 300 bp long promoter sequence contains a RBS and a FUR binding site, although it has not been clearly possible to identify them.


Characterization:
We characterized its inhibition ability by cloning a superfolder GFP downstream at a iron concentration range from 10-7 to 10-4 mol.L-1.


Why would you use this part?
This part should be used for iron sensing purposes in Escherichia coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]