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| − | __NOTOC__
| + | #REDIRECT [[Help:An Introduction to BioBricks]] |
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| − | |[[image:Partinplasmid.png]]
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| − | BioBricks are a set of interchangable parts, designed with a view to building biological systems in living cells. The picture to the left shows BioBrick part [[Part:BBa_B0034|BBa_0034]] within a [[Help:Plasmids|plasmid]]. The yellow arrow represents the plasmid's antibiotic resistance gene and the green arrow, its origin of replication. For more information see:
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| − | *[[Part Names|BioBrick Names]]
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| − | *[[Part Types|Types of BioBrick]]
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| − | *[[Help:Plasmids/Construction Plasmids|Plasmids Introduction]]
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| − | |}
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| − | ==Building BioBrick Systems==
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| − | |[[image:MultiPartsinplasmid.png]]
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| − | BioBrick parts can be assembled to form useful devices, through a process often refeed to as 'Standard Assembly' This uses normal cloning techniques based on [[Assembly:Restriction enzymes|restriction enzymes]], purification, ligation, and transformation. BioBrick parts are [[Assembly:Composable|composable]]; allowing endless numbers of biobricks to be pieced together to form higher systems.
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| − | The assembly to the left shows 3 BioBricks assembled to create a simple device. You can learn more about this device by following the link to its associated page in the registry. You may like look into:
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| − | *[[Assembly:Standard assembly|Standard Assembly]] - <i>The classical method of BioBrick assembly, done at the lab bench</i>
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| − | *[[Assembly:Restriction enzymes|BioBrick Restriction Enzymes]]
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| − | *[[Assembly:Rolling assembly|Rolling Assembly]]
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| − | *[[Assembly:Robotic assembly| Automated assembly]] - <i>currently in late stage development at iGEM labs</i>
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| − | ==How do I make a BioBrick?==
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| − | ====At the bench====
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| − | Pic of biobrick flanked by coding seq
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| − | Methods:
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| − | *Synthesis
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| − | *PCR
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| − | More information on putting sequence into standardized Biobrick form can be found [[Help:Standardization|here]]
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| − | ====Adding the part to the registry====
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| − | This protocol is quick and easy. A comprehensive guide can be found [[Basic Biobrick, example 1|here]]
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