Difference between revisions of "Part:BBa K1197009"

 
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1197009 short</partinfo>
 
<partinfo>BBa_K1197009 short</partinfo>
 +
 +
This part is used together with K119008 and K1197010 to compare two Pveg promoter (K143012 and K823003) and one pHyperspank promoter (K606040) which used in B.subtilis and have different sequences. By RFP production and modelling data, strength of promoters were compared.
  
 
.
 
.
Line 11: Line 12:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1197009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1197009 SequenceAndFeatures</partinfo>
 +
 +
We simulated the production of RFP under two different IPTG concentrations to observe its basal and maximum transcription rates.
 +
 +
 +
'''IPTG = 0'''
 +
 +
https://static.igem.org/mediawiki/parts/7/75/K606040_-_IPTG_%3D_0.jpg
 +
 +
As seen in the graph, when there is no IPTG with the increasing LacI expression, the RFP amount becomes constant and this means there is no more production of RFP.
 +
 +
'''IPTG = 5000'''
 +
 +
https://static.igem.org/mediawiki/parts/b/b6/K606040_-_IPTG_%3D_5000.jpg
 +
 +
As seen in this graph, with 5000 IPTG molecules, LacIs form complexes with IPTG. Therefore there is decreased inhibition on K606040 and RFP production continues and the amount keeps getting higher.
  
  

Latest revision as of 03:45, 5 October 2013

Phyp + RBS (SpoVG) + RFP + DT

This part is used together with K119008 and K1197010 to compare two Pveg promoter (K143012 and K823003) and one pHyperspank promoter (K606040) which used in B.subtilis and have different sequences. By RFP production and modelling data, strength of promoters were compared.

.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 673
    Illegal AgeI site found at 785
  • 1000
    COMPATIBLE WITH RFC[1000]

We simulated the production of RFP under two different IPTG concentrations to observe its basal and maximum transcription rates.


IPTG = 0

K606040_-_IPTG_%3D_0.jpg

As seen in the graph, when there is no IPTG with the increasing LacI expression, the RFP amount becomes constant and this means there is no more production of RFP.

IPTG = 5000

K606040_-_IPTG_%3D_5000.jpg

As seen in this graph, with 5000 IPTG molecules, LacIs form complexes with IPTG. Therefore there is decreased inhibition on K606040 and RFP production continues and the amount keeps getting higher.