Difference between revisions of "Part:BBa K1017402"
 
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The DNA sequence can produce sRNA and combine with the sequence of its target region on the rRBS-2 designed by us, blocking the gene expressions downstream. It is the temporary design which is simply amplified.
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The DNA sequence produces sRNA which can combine with the sequence of its target region on the rRBS-2 designed by us, blocking the gene expressions downstream. It is the temporary design which can be simply amplified.
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[[File:NCTU sRNA2-synthesis process.png|center|600px]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:34, 26 October 2013

sRNA+rRBS-2


The DNA sequence produces sRNA which can combine with the sequence of its target region on the rRBS-2 designed by us, blocking the gene expressions downstream. It is the temporary design which can be simply amplified.

NCTU sRNA2-synthesis process.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 97
    Illegal SpeI site found at 82
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 82
    Illegal NotI site found at 88
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 97
    Illegal SpeI site found at 82
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 97
    Illegal SpeI site found at 82
  • 1000
    COMPATIBLE WITH RFC[1000]