Difference between revisions of "Part:BBa K1093016"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1093016 short</partinfo> | <partinfo>BBa_K1093016 short</partinfo> | ||
| − | This is N- | + | This is the N-terminus of mCherry, that can be used in Bimolecular Fluorescence Complementation system (BiFC). Split between amino acids 159 and 160. Emission/excitation maxima are 587/610 nm. Known to have short maturation time and can be potentially observed with naked eye.Alongside BBa_K1093017 this part can be used for investigating protein-protein interactions. |
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| + | ==References== | ||
| + | 1: Fan JY, Cui ZQ, Wei HP, Zhang ZP, Zhou YF, Wang YP, Zhang XE. Split mCherry as | ||
| + | a new red bimolecular fluorescence complementation system for visualizing | ||
| + | protein-protein interactions in living cells. Biochem Biophys Res Commun. 2008 | ||
| + | Feb 29;367(1):47-53. Epub 2007 Dec 26. PubMed PMID: 18158915. | ||
| + | |||
| + | 2: Kodama Y, Hu CD. Bimolecular fluorescence complementation (BiFC): a 5-year | ||
| + | update and future perspectives. Biotechniques. 2012 Nov;53(5):285-98. doi: | ||
| + | 10.2144/000113943. Review. PubMed PMID: 23148879. | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 18:37, 12 October 2013
mCherry N-terminal
This is the N-terminus of mCherry, that can be used in Bimolecular Fluorescence Complementation system (BiFC). Split between amino acids 159 and 160. Emission/excitation maxima are 587/610 nm. Known to have short maturation time and can be potentially observed with naked eye.Alongside BBa_K1093017 this part can be used for investigating protein-protein interactions.
References
1: Fan JY, Cui ZQ, Wei HP, Zhang ZP, Zhou YF, Wang YP, Zhang XE. Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein-protein interactions in living cells. Biochem Biophys Res Commun. 2008 Feb 29;367(1):47-53. Epub 2007 Dec 26. PubMed PMID: 18158915.
2: Kodama Y, Hu CD. Bimolecular fluorescence complementation (BiFC): a 5-year update and future perspectives. Biotechniques. 2012 Nov;53(5):285-98. doi: 10.2144/000113943. Review. PubMed PMID: 23148879.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]