Difference between revisions of "Part:BBa K1154001:Design"

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===Design Notes===
 
===Design Notes===
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This sequence is from ''Saccharomyces cerevisiae'' Reference Strain S288C. It is very similar to the FUS1 promoter well characterized by the 2012 TU-Delft iGEM Team (see [https://parts.igem.org/Part:BBa_K775004 Part:BBa_K775004]). However, the sequences do differ:
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Sequence is from ''Saccharomyces cerevisiae'' Reference Strain S288C chromosome III coordinates 71603 to 71803, in the promoter region of the FUS1/YCL027W coding sequence. It is very similar to the FUS1 promoter well characterized by the 2012 TU-Delft iGEM Team (see [https://parts.igem.org/Part:BBa_K775004 Part:BBa_K775004]). However, the two sequences do differ:
  
 
[[File:Align_BBa_K775004_BBa_K1154001.JPG]]
 
[[File:Align_BBa_K775004_BBa_K1154001.JPG]]
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===Source===
 
===Source===
  
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Sequence is from the genome of ''Saccharomyces cerevisiae'' Reference Strain S288C chromosome III coordinates 71603 to 71803, in the promoter region of the FUS1/YCL027W coding sequence ([http://www.yeastgenome.org ''Saccharomyces'' Genome Database]).
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Sequence is from the genome of ''Saccharomyces cerevisiae'' Reference Strain S288C.
  
 
===References===
 
===References===
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McCaffrey G, Clay FJ, Kelsay K, and Sprague GF Jr (1987) Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 7 (8), 2680-2690.
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Please consult the [http://www.yeastgenome.org/ ''Saccharomyces'' Genome Database] for copius information and references.

Latest revision as of 02:59, 21 September 2013

Saccharomyces cerevisiae FUS1 promoter (induced by mating pheromone)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence is from Saccharomyces cerevisiae Reference Strain S288C chromosome III coordinates 71603 to 71803, in the promoter region of the FUS1/YCL027W coding sequence. It is very similar to the FUS1 promoter well characterized by the 2012 TU-Delft iGEM Team (see Part:BBa_K775004). However, the two sequences do differ:

Align BBa K775004 BBa K1154001.JPG

Source

Sequence is from the genome of Saccharomyces cerevisiae Reference Strain S288C.

References

Please consult the [http://www.yeastgenome.org/ Saccharomyces Genome Database] for copius information and references.