Difference between revisions of "Part:BBa K1130000:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1130000 short</partinfo> | <partinfo>BBa_K1130000 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | Biobricks prefix and subffix was add to the sequence | + | Biobricks prefix and subffix was add to the sequence. |
| − | + | Synthetic Promoter derived from rRNA promoters extracted from the L. plantarum WCFS1 genome. | |
| − | + | ||
===Source=== | ===Source=== | ||
Synthetic Promoter derived from rRNA promoters extracted from the L. plantarum WCFS1 genome. | Synthetic Promoter derived from rRNA promoters extracted from the L. plantarum WCFS1 genome. | ||
| + | Cloned and expression in L.plantarum and E.coli, measured activity with GFP ligation | ||
| + | [[File:P1 expression.jpg]] | ||
===References=== | ===References=== | ||
| + | Rud I, Jensen PR, Naterstad K, Axelsson L. (2006) A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum. | ||
Latest revision as of 22:07, 5 October 2013
Constitutive promoter 1 L.plantarum
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Biobricks prefix and subffix was add to the sequence. Synthetic Promoter derived from rRNA promoters extracted from the L. plantarum WCFS1 genome.
Source
Synthetic Promoter derived from rRNA promoters extracted from the L. plantarum WCFS1 genome.
Cloned and expression in L.plantarum and E.coli, measured activity with GFP ligation
References
Rud I, Jensen PR, Naterstad K, Axelsson L. (2006) A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum.