Difference between revisions of "Part:BBa K1022124"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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+ | ==Information== | ||
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+ | This part is one of the final constructs designed in the project '''[http://2013.igem.org/Team:TU-Delft/Project Peptidor]''' by Team TU Delft | ||
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+ | '''Please visit [http://2013.igem.org/Team:TU-Delft iGEM 2013 Team TU Delft] for more''' | ||
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Latest revision as of 16:08, 4 October 2013
pT7: RBS: His6- SUMO: MaximinH5: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Lysis
This biobrick is the one of the three final constructs of the project Peptidor by Team TU Delft iGEM 2013. This has a Promoter pT7 along with a His6 SUMO tagged peptide followed by a TetR which suppresses the timer module containing promoter pTET, cI is produced which suppresses pcI. Then Ulp protease is produced following by a Kill switch lysis device.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 46
Illegal NheI site found at 2361
Illegal NheI site found at 4456
Illegal NheI site found at 4479
Illegal NotI site found at 3057 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 170
Illegal BamHI site found at 3026
Illegal XhoI site found at 3035
Illegal XhoI site found at 3066 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 355
Illegal AgeI site found at 4061
Illegal AgeI site found at 4131 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 4712
Information
This part is one of the final constructs designed in the project [http://2013.igem.org/Team:TU-Delft/Project Peptidor] by Team TU Delft
Please visit [http://2013.igem.org/Team:TU-Delft iGEM 2013 Team TU Delft] for more